摘要
目的:观察蓝布正提取物(GHE)对血管性痴呆(VD)大鼠学习记忆能力的影响并探讨其作用的机制。方法:采用双侧颈总动脉结扎法制作VD大鼠模型,将大鼠随机分成假手术组,模型组,GHE高、中、低剂量组(7,3.5,1.75 g·kg^(-1))和阳性药组(天保宁银杏叶片,0.007 g·kg^(-1)),各组灌胃相应药物,每日1次,连续35 d,在第30~35天进行Morris水迷宫实验检测大鼠的学习记忆能力,Morris水迷宫实验结束后处死大鼠,收集大脑样本,免疫组化法检测海马CA1区B细胞淋巴瘤-2(Bcl-2)相关X蛋白(Bax),Bcl-2,神经营养因子-3(NT-3),脑源性神经营养因子(BDNF)蛋白表达。结果:与模型组比较,GHE高、中剂量组可明显缩短大鼠的逃避潜伏期,增加穿越平台数(P<0.05);GHE高、中、低剂量组可显著降低海马CA1区Bax表达,升高Bcl-2,NT-3,BDNF表达(P<0.05,P<0.01)。结论:GHE能改善VD大鼠的学习记忆能力,其机制可能与促进海马CA1区NT-3,BDNF的表达,抑制神经细胞凋亡有关。
Objective： To explore the protective effect and mechanism of Gei Herba extract （GHE） on memory and learning ability of rats model of vascular dementia （VD）. Method： Rats with vascular dementia were prepared with bilateral carotid artery ligation. Then the rats were randomly divided into 6 groups, namely sham operation group, model group, GHE high, medium and low dose groups （7, 3.5, 1.75 g·kg^-1 ） , and Taponing positive control group （0. 007 g·kg^-1）. The corresponding drug was given by oral administration for 35 d, once a day. The behavior tests were carried out by water maze task on day 30-35 to test the memory and learning abilities of the rats, then they were sacrificed at the end of water maze test. The protein expression levels of B-cell lymphoma-2 （Bcl-2） assolated X protein （Bax）, Bcl-2, nerve neurotrophic factor-3 （NT-3） and nerve neurotrophic factor-3 （BDNF） in hippocampal CAa region of brain samples collected after the last administration were detected by immunocytochemistry. Result： GHE high and middle dose groups can significantly shorten the escape latency and increase the number of cross platforms （P 〈 0.05） ; GHE high, middle and low dose groups can significantly decline the expression levels of Bax and rise the expression levels of Bcl-2, NT-3 and BDNF （P 〈 0.05 , P 〈0.01 ）. Conclusion： GHE can improve learning and memory abilities of VD rats, and its mechanism may be related to the promotion of NT-3 and BDNF expression in hippocampal CA1 region and the inhibition of neurons apoptosis.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第17期154-158,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技合作计划项目(黔科合LH字[2015]7274号)
贵州省科技计划项目(黔科合院士站[2014]4013号)
贵州省协同创新中心建设项目(黔教合协同创新字[2013]02号)
贵州省普通高等学校工程研究中心建设项目(黔教合KY字[2014]22号)
