摘要
目的了解广东地区人群抗-Mur发生频率、性质和来源以及Mur抗原的分布;建立献血者Mur稀有血型库服务于临床用血。方法以微量板的盐水法和聚凝胺法对广东省中山、深圳、江门、东莞、惠州市34 419名献血者做抗-Mur筛查,用抗-Mur做红细胞Mur抗原筛查,部分标本采用固相免疫吸附试验(SPIA)筛查。结果本组筛查对象共检出抗-Mur 48例,占0.14%(48/34 419),其中IgM占79.2%(38/48),IgM+IgG占16.7%(8/48),IgG占4.2%(2/48);献血者抗-Mur频率比较,无显著性差异(P>0.05);中山患者抗-Mur频率为0.17%(5/3010),与献血者比较无明显差异(P>0.05);中山地区,Mur抗原在献血者、患者中的发生率分别为7.31%(128/1 752)vs 7.14%(80/1 120),P>0.05,献血者男性和女性中分别为7.7%(72/931)vs 6.5%(33/504),P>0.05;本组Mur阳性者均为汉族,其中广东省籍贯占66.7%(70/105);建立了中山地区献血者Mur稀有血型库;SPIA技术从2 541份血浆标本中检出4例抗-Mur。结论 SPIA可以检出37℃活性有较高临床意义的抗-Mur,广东地区献血人群Mur血型抗体、抗原发生频率均明显高于Rh D血型抗体、抗原发生频率,不规则抗体检测应增加Mur阳性筛选红细胞,供受血者均应检测Mur血型是否相合,以进一步提高输血免疫安全性。
Objective To investigate the frequency of anti-Mur antibodies in the population of Guangdong province,analyze the properties and sources of these antibodies and establish Mur blood bank for clinical use.Methods Anti-Mur antibodies were screened by the saline agglutination test and the polybrene method in microplate.Mur antigen RBCs phenotypes were detected with anti-Mur antibodies by the saline agglutination test and the polybrene method in microplate.Part of samples were detected by solid phase immune adsorption test（SPIA）.Results 34 419 samples of blood donors from 5 cities（Zhongshan,Shenzhen,Jiangmen,Dongguan,Huizhou） in Guangdong province were detected and 48 cases were anti-Mur positive（0.14%,48/34 419）.The proportion of Ig M was 79.2%（38/48）,Ig M＋Ig G was 16.7%（8/48）,Ig G was 4.2%（2/48）.There was no significant difference in anti-Mur frequency of blood donors among the 5 cities,so did anti-Mur frequency between the patients and blood donors.The total positive rate of Mur antigen was 7.24%（208/2 872）,including7.31% in blood donors（128/1 752） and 7.14% in patients（80/1 120）（P〉0.05）.The Mur positive people i were all Han people,66.7% of examinee＇s native place were in Guangdong.We established Mur blood bank.Four cases of anti-Mur were detected from 2 541 blood samples with SPIA method.Conclusion Anti-Mur antibodies has clinical significance at 37 degrees,and they could be detected with SPIA method.The frequencies of anti-Mur antibodies and Mur antigens were significantly higher than that of anti-D antibody incidence and Rh D antigen negative blood types in the population of Guangdong area.Mur blood group should be examined and Mur positive panel red blood cells should be used in irregular antibody detection,in order to improve the safety of transfusion.
出处
《中国输血杂志》
北大核心
2017年第6期586-589,共4页
Chinese Journal of Blood Transfusion
基金
中山市科技计划项目(2014A1FC045)
江苏省自然科学基金(BK20130343)
