摘要
目的:研究鼠源性神经生长因子联合早期亚低温治疗对重型颅脑外伤患者的脑保护作用及对炎症、应激反应指标的影响。方法:收集本院收治的重型颅脑外伤患者68例,回顾治疗过程、实验室检查结果,分为接受亚低温治疗的对照组38例,接受鼠源性神经生长因子联合早期亚低温治疗的观察组30例。入院后即刻、治疗2周后,采用全自动生化分析仪检测神经损伤指标含量;采用酶联免疫吸附法(ELISA)检测神经递质、炎症因子含量;采用放射免疫法检测应激激素含量。结果:治疗前,两组神经损伤指标、神经递质、炎症因子、应激激素的差异无统计学意义(P>0.05);治疗后,观察组外周血神经功能指标MBP、IMA、NSE含量低于对照组,BDNF含量高于对照组(P<0.05);观察组外周血肽类神经递质VAP、Dny-A、NPY及氨基酸类神经递质Glu、Asp含量低于对照组,GABA含量高于对照组(P<0.05);观察组血清中炎症因子CRP、NF-κB、IL-1β含量以及应激激素AngⅡ、Cor、CRH、NE含量低于对照组(P<0.05)。结论:鼠源性神经生长因子联合早期亚低温治疗能够保护重型颅脑外伤患者的脑功能,抑制全身炎症及应激反应。
Objective：To study the cerebral protective effect of mouse nerve growth factor combined with early hypothermia therapy for patients with severe craniocerebral injury and the influence on the inflammatory and stress reaction indexes.Methods：A total of 68 patients with severe craniocerebral injury who were treated in our hospital between May 2012 and February 2016 were collected and then divided into the control group（n=38）who received mild hypothermia therapy and the observation group（n=30）who received mouse nerve growth factor combined with early hypothermia therapy after the treatment and laboratory test results were reviewed.Immediately after admission and after 2weeks of treatment,automatic biochemical analyzer was used to detect the contents of nerve injury indexes;enzyme-linked immunosorbent assay（ELISA）was used to detect the contents of neurotransmitters and inflammatory factors;RIA method was used to detect the contents of stress hormones.Results：Before treatment,the nerve injury indexes,neurotransmitters,inflammation factors and stress hormones were not statistically different between two groups of patients（P〉0.05）;after treatment,peripheral blood nerve function indexes MBP,IMA and NSE levels of observation group were lower than those of control group while BDNF level was higher than that of control group patients（P〈0.05）;peripheral blood peptide neurotransmitters VAP,Dny-A and NPY as well as amino acid neurotransmitters Glu and Asp contents of observation group were lower than those of control group while GABA level was higher than that of control group（P〈0.05）;serum inflammatory factors CRP,NF-κB and IL-1βcontents as well as stress hormones AngⅡ,Cor,CRH and NE contents of observation group were lower than those of control group（P〈0.05）.Conclusions：Mouse nerve growth factor combined with early hypothermia therapy can protect the brain function and inhibit the systemic inflammatory and stress reaction in patients with severe craniocerebral injury.
出处
《海南医学院学报》
CAS
2017年第7期958-961,965,共5页
Journal of Hainan Medical University
基金
重庆市黔江区科技计划项目(黔科技2013016)~~
关键词
颅脑外伤
鼠源性神经生长因子
亚低温
炎症反应
应激反应
Craniocerebral injury
Mouse nerve growth factor
Hypothermia
Inflammatory reaction
Stress reaction
