摘要
构建组织工程化软骨常常需要体外扩增软骨细胞。骨但是,软骨细胞在体外培养、扩增过程中常发生去分化（（dedifferentiation）而丧失细胞表型,导致再生软骨中1II型胶原、氨基葡萄糖（GAG）合成减少,易发生退变。区目前有关去分化的具体机理尚未阐明,但以往的研究表短明去分化主要与体外扩增的软骨细胞缺乏细胞与细胞外结基质（extracellular matrix,ECM）之间有效的信号刺激有高关[2-3]。
Objective In order to provide a better way to increase the quality of tissue-engineered cartilage, the effects of integrin on the dedifferentiation of chondrocyte were reviewed. Methods By searching PubMed and CBM, we reviewed published papers on integrin and chondrocyte dedifferentiation and analyzed the effects of integrin. Results （ 1 ） Integrin is a transmembrane glycoprotein on the surface of chondrocyte, belonging to the family of cell adhesion molecules. As the surface receptor of chondrocyte, it plays an important role in the interaction between the cell and extracellular matrix （ ECM ）. （ 2 ） Integrin, which can be combined with a variety of signaling protein in the ECM, will transmit the stimulus signals to the cytoskeletal （ CSK ） and cytosolic proteins. Additionally, it can regulate the function of chondrocyte and affect the dedifferentiation through multiple pathways. （ 3 ） As a bridge between CSK and ECM, there are a number of critical junctions in the integrin which ensure normal operation of the signal of ECM- Integrin-CSK. （ 4 ） Because of the structural characteristics, a unified system of ECM and the intracellular protein are linked by integrin, which can be used to mediate the stimulation and regulate the function of chondrocyte. Conclusions Chondrocyte dedifferentition is closely related to integrin, which can affect cell viability, cell adhesion and cell phenotype. Due to the transmition of ECM, it can regulate the chondrocyte dedifferentiation process through specific singling pathway.
作者
张煜
刘鑫成
范宏斌
ZHANG Yu-shen LIU Xin-eheng FAN Hong-bin.(Institute of Orthopedics, Xijing Hospital, the fourth Military Medical University, Xi ＇an, Shanxi, 710032, China)
出处
《中国骨与关节杂志》
CAS
2017年第3期232-236,共5页
Chinese Journal of Bone and Joint
基金
国家自然科学基金项目(31170936
31470936)
关键词
整合素类
软骨细胞
细胞去分化
软骨
Integrins
Chondrocytes
Cell dedifferentiation
Cartilage
