摘要
采用形态学、分子生物学和柯赫氏法则验证技术,对引起内蒙古甘薯根腐病的腐霉菌进行了鉴定。2012年从内蒙古通辽市科尔沁左翼后旗甘薯种植区采集的病样中分离获得45个菌株,经形态学观察和rDNA-ITS序列分析,确定其中43个菌株为Pythium ultimum var.ultimum,分离频率高达95.6%。致病性测定结果表明,回接后观察到与田间自然发病症状相同,并从发病根部重新分离到初始接种的腐霉菌株。腐霉菌引起甘薯根腐病在内蒙古为首次报道。
Pathogen of sweet potato root rot was identified by morphology, rDNA-ITS sequence analysis and Koch＇s postu- lates in Inner Mongolia. 43 strains of 45 Pythium isolated from the diseased sweet potato collected from Keerqinzuoyihouqi county of Tongliao city, Inner Mongolia in 2012 were identified as P. ultimum var. ultimum by morphological characteristics and rITS sequence analysis, and isolation rates reached to 95.6%. After inoculation, the isolates could infect sweet potato and showed the same symptom, and the same isolates were re-isolated from inoculated roots. This was the first report of pythium causing root rot of sweet potato in Inner Mongolia.
出处
《中国植保导刊》
北大核心
2017年第2期10-14,共5页
China Plant Protection
基金
国家自然科学基金(31160353
31360574)
通辽市与内蒙古民族大学科技合作项目(SXYB2012063)
关键词
甘薯
腐霉菌
致病性
sweet potato
Pythium spp.
pathogenicity
rDNA-ITS
