摘要
目的观察和营安心方对衰老大鼠血清和脑组织白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的影响,探讨和营安心方对脑组织的保护作用。方法 48只Wistar雄性大鼠随机分为正常组、模型组、维生素E组及和营安心方高、中、低剂量组。除正常组外,其他组颈背部皮下注射D-半乳糖125 mg·kg^(-1)·d^(-1)制备大鼠衰老模型,各组给予相应药物灌胃,正常组和模型组给予等量生理盐水灌胃,连续8周。酶联免疫吸附法(ELISA)检测各组大鼠血清和脑组织中IL-6和TNF-α含量。结果与正常组比较,模型组血清和脑组织中IL-6和TNF-α含量增高,差异有统计学意义(P<0.05);与模型组比较,各给药组血清和脑组织中IL-6和TNF-α含量降低,差异有统计学意义(P<0.05),和营安心方高剂量组作用最为显著。结论和营安心方可下调血清和脑组织中IL-6和TNF--α的含量,通过减轻炎症反应,对脑组织起到保护的作用。
Objective To observe effects of He Ying An Xin Fang on IL-6 and TNF-a in Serum andbrain of aging rats and explore its protective effects on brain. Methods 48 Wistar male rats were random-ly divided into normal group, model group, He Ying An Xin Fang high-dose group, He Ying An XinFang mid-dose group, He Ying An Xin Fang low-dose group and Vitamin E group. Except the normalgroup, the other groups were injected with D-galactose at 125 mg （kg ·d） in the back of the neck to pre-pare the rat model of aging. All groups were given the corresponding doses, while normal and modelgroup everyday received gavage by giving the same amount of saline for 8 weeks. Then the IL-6 and TNF- in serum and brain were detected with ELISA. Results Compared with normal group, IL-6 and TNF-alevels in serum and brain tissue in model groups were increased with statistical significance （P〈0. 05）.Compared with model group, levels of IL-6 and TNF-a in other groups were decreased with statistical sig-nificance （P〈0.05）. And among all the groups, the He Ying An Xin Fang high-dose group has the mostobvious effect. Conclusion He Ying An Xin Fang could down-regulate the level of IL-6 and TNF-a in ser-um and brain tissue. By reducing level of pro-inflammatory factors, He Ying An Xin Fang could have pro-tective effect on brain tissue.
出处
《河北北方学院学报（自然科学版）》
2016年第12期1-3,共3页
Journal of Hebei North University:Natural Science Edition
基金
河北北方学院创新人才培育基金课题(No.CXRC1319)
