摘要
目的分析重组戊型肝炎疫苗P179抗原蛋白颗粒及二聚体在不同条件下的稳定性,为该抗原的纯化工艺及原液储存条件提供参考。方法将重组戊型肝炎疫苗原液置不同温度、不同p H、高盐浓度、高盐低p H条件下及低温长期储存后,通过透射电镜观察P179抗原蛋白颗粒状态,SDS-PAGE分析二聚体含量。结果 HEV抗原在不同温度、不同p H、高盐浓度、高盐低p H条件下,及低温储存不同时间,P179抗原蛋白颗粒维持在20 nm左右,二聚体含量不低于80%;P179抗原低温储存24个月,其蛋白颗粒及二聚体含量均未发生明显改变。结论重组戊型肝炎疫苗P179抗原蛋白颗粒及二聚体稳定性较好。
Objective To analyze the stability of P179 antigen protein particles and dimer of recombinant hepatitis E vaccine（r HEV） under various conditions and provide a reference for the purification and storage of the bulk of antigen.Methods Bulk of r HEV antigen was stored at various temperatures and p H values, at high salt concentration, at high salt concentration plus low p H value, and at low temperature, observed for protein particles by transmission electron microscopy, and determined for dimer content by SDS-PAGE. Results After storage at various temperatures and p H values, high salt concentration, high salt concentration plus low p H value and low temperature for various time periods, the sizes the P179 antigen protein particles were maintained at about 20 nm, while the dimer content was not less than 80%.However, after storage at low temperature for 24 months, neither size of antigen protein particles nor dimer content showed significant change. Conclusion Both P179 antigen protein particles and dimer of r HEV showed high stability.
出处
《中国生物制品学杂志》
CAS
CSCD
2016年第12期1233-1237,共5页
Chinese Journal of Biologicals
基金
吉林省产业技术研究与开发项目(200903)
长春市科技计划项目(11KZ79)
