摘要
为加强野生湖北百合资源保存与利用,选用黔东南州野生湖北百合为材料,选用鳞茎及茎段为外植体,探讨不同浓度激素配比对其不定芽诱导、增殖及生根的影响。结果表明:诱导不定芽的最佳培养基为MS+1.0mg·L-1 6-BA+0.5mg·L-1 NAA,但茎段诱导不定芽高于鳞片,出芽率分别为66.67%和59.63%,单块外植体出芽个数分别为2和1,且茎段污染率比鳞片的污染率低。茎段诱导最佳增殖培养基为MS+0.1mg·L-16-BA+1.0mg·L-1 NAA,增殖率为83.33%,平均增殖系数为2.11,其生长状况良好,为最佳增殖培养基。茎段诱导最佳生根培养基为1/2MS+0.3mg·L-1 NAA,诱导生根率高达93.33%,平均生根数为3.18。
In order to strengthen the preservation and utilization of resources of wild Liliurn henryias, the effects of different hormone combination on adventitious buds induction, bud proliferation and rooting were explored by using bulb and stem of wild Lilium henryias as explant. The results showed that the optimal medium for ad- ventitious buds induction was MS＋1.0 mg·L^-1 6-BA＋0.5 mg·L^-1 NAA. The induction rate of stem was higher than bulbs. The rate of induction was 66.67 % and 59.63 %. The bud number from single explant was 2 and 1,the rate of contamination from stem was lower than from bulb. The best proliferation medium was MS ＋ 0.1 mg·L^-1 6-BA ＋1.0 mg·L^-1 NAA. The rate of proliferation was 83.33% ,the average of coefficient of proliferation was 2.11 and the growth was better. The medium for rooting was 1/2MS＋0.3 mg·L^-1 NAA. The rate of rooting was as high as 93. 33%, the average number of rooting was 3. 18. The theoretical base is the germplasm resource of wild Lilium henryi for breeding and conserving.
出处
《黑龙江农业科学》
2016年第10期13-16,共4页
Heilongjiang Agricultural Sciences
基金
贵州省科技厅资助项目(黔科合J字[2014]2153号)
凯里学院博士启动基金资助项目(BS201332)
