摘要
目的研究游离微小RNA-198（miR-198）对肺癌相关的恶性胸腔积液的诊断价值。方法采用实时荧光定量PCR方法检测54例肺癌恶性胸腔积液和35例良性胸腔积液中胸液游离miR-198的相对表达水平。结果肺癌恶性胸腔积液miR-198的表达量小于良性胸腔积液,差异有统计学意义（P〈0.01）。ROC曲线显示miR-198能够较好地区分良恶性胸腔积液,曲线下面积（AUC）为0.899（95%CI：0.822~0.950）,优于CEA的0.774（95%CI：0.716~0.825）。当miR-198和CEA联合检测时,敏感度增高到85.7%,特异度为90.9%,AUC升高到0.920（95%CI：0.844~0.963）。游离miR-198表达水平与MPE患者年龄、性别、吸烟史、肿瘤类型、肿瘤转移位置以及CEA的水平无关（P〉0.05）。结论胸液游离miR-198可作为肺癌恶性胸腔积液诊断的分子标志物。
Objective To evaluate the diagnostic value of cell- free miR- 198 in the diagnosis of lung cancer- associated malignant pleural effusions（ MPEs）. Methods The cell- free levels of miR- 198 from 54 MPEs and 35 benign pleural effusions（ BPEs） were determined using a real- time reverse transcription quantitative polymerase chain reaction. Results The expression level of miR- 198 was significantly lower in the patients with MPEs than the patients with BPEs,with the difference statistically significant（ P〈0. 01）. ROC curve showed that miR- 198 can better distinguish between benign and malignant pleural effusion,the area under the curve（ AUC） was 0. 899（ 95% CI： 0. 822- 0. 950）,which was better than 0. 774（ 95% CI：0. 716- 0. 825） of CEA. When miR- 198 and CEA were combined,the sensitivity increased to 85. 7%,the specificity was90. 9%,and the AUC increased to 0. 920（ 95% CI： 0. 844- 0. 963）. Free miR- 198 expression levels was not correlative to age,sex,smoking history,tumor type,tumor metastasis,and CEA levels in patients with MPE（ P〉0. 05）. Conclusion Cell- free miR- 198 in patients could be used as a new biomarker for the diagnosis of lung cancer- malignant pleural effusion.
出处
《中国卫生检验杂志》
CAS
2016年第21期3053-3056,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江省医药卫生科技计划项目(2014KYB233)
宁波市自然科学基金项目(2012A610194)
