摘要
目的:在亲子鉴定案件中检出D6S1043基因座三带型。方法:提取个体不同组织DNA样本,经复合荧光PCR扩增和毛细管电泳分析2716个案例(共6246名个体)的STR分型。结果:在1个父子单亲鉴定案例中,2名个体的D6S1043基因座上出现三带型等位基因。用Power Plex?21荧光标记复合扩增试剂盒对包含D6S1043在内的21个STR基因座进行检测,父亲D6S1043基因座分型为17/18/21,儿子分型为10/17/18,电泳峰高和峰面积之比均为1∶1∶1,用SinofilerTM试剂盒复核检测结果相同。结论:在本文发现的案例中,父亲的等位基因17和18均遗传给了儿子,导致儿子表现为三带型等位基因。D6S1043三带型非常罕见,在分析时应采用不同方法或者试剂盒进行验证,保证鉴定结果的可靠性。
Aim： To report a three-banded allele pattem of the STR locus D6S 1043 in a paternity identification. Methods： DNA was extracted from different tissue samples of the individual. STR genotype of 2716 cases （6246 individuals） were determined and analyzed by multiplex fluorescent PCR amplication and capillary electrophoresis. Results： Two cases were confirmed to have three-banded allele locating in D6S 1043 locus in a father-child case. PowerPlex 21 multiplex fluorescent PCR amplication system detected 21 STR loci including D6 S 1043 locus. Father＇s alleles in D6S 1043 locus were 17/18/21.Child＇s alleles in D6S 1043 locus were 10/17/18, the height and area ratio in D6S 1043 locus both were about 1：1：1. The result of three-banded allele of D6S 1043 locus was the same as SinofllerTM kit. Conclusions： In this case father＇s 17 and 18 alleles passed on to his son leading to the three-banded allele pattern. The three-banded allele pattern of the STR locus D6S1043 is rare. To ensure the reliability of analysis results, different methods should be adopted for validation.
出处
《大众科技》
2016年第7期87-88,共2页
Popular Science & Technology
