摘要
目的:探讨二乙基二硫代氨基甲酸酯(DDC)对婴儿双歧杆菌超氧化物歧化酶(SOD)活性的作用。方法:采用厌氧活化扩增法培养婴儿双歧杆菌,根据绘制出的最适生长曲线,在平台生长期内加入不同浓度(200、1 000μmol/L)的DDC作用一段时间(0、0.5、1、2、4、24 h),然后离心收集细菌、进行超声破碎,测定细菌裂解液中SOD的活性。作为阳性对照,我们也检测了DDC对小鼠肠道裂解液中SOD活性的影响。结果:我们发现上述不同浓度的DDC作用于婴儿双歧杆菌一定时间后,对其SOD活性无显著影响。动物实验表明DDC作用于小鼠一定时间后,与对照组相比,其SOD活性显著降低(P<0.01)。结论:DDC影响小鼠体内SOD活性,但不影响婴儿双歧杆菌SOD的活性。推断婴儿双歧杆菌SOD抑制剂不是DDC,SOD抑制剂尚需进一步研究。
Objective： To investigate whether diethyldithiocarbamate（ DDC） affect the activity of superoxide dismutase（ SOD）of Bifidobacterium infantis（ BB）. Methods： We used simple anaerobic activated ways to culture BB. After the bacterium reached platform growth period,two concentrations（ 200 and 1 000 μmol / L） of DDC were added to bacterium medium for a specific period of time（ 0. 5,1,2,4 and 24 h）,which was in order to determine if this inhibitor affected the activity of SOD. As a positive control,the inhibitory effect of DDC on the activity of SOD in murine intestinal samples was also checked. Results： It was found that incubation of BB with DDC for some specific periods showed no effect on the activity of SOD. However,the activity of SOD in intestine reduced dramatically after the mice were given DDC in vivo. Conclusion： DDC affect the SOD activity in mice,while it showed no effect on SOD activity of BB. The suitable inhibitor for SOD of BB is not commercially available and further research is warranted.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第8期1103-1106,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(81300292)
广东省对外科技合作项目(No.2013B051000088)
深圳市科技计划国际科技合作项目(No.GJHZ20130408174112021)
深圳市科技计划基础研究项目(No.JCYJ20140418095735538
JCYJ20140828163633991)
