摘要
本试验采用国内分离的双歧双歧杆菌菌株Ｂｉｆ１１０１，用ＴｒｉｔｏｎＸ－１００处理ＢＬ肉汤培养物，高速离心分离不可溶沉渣，经蛋白酶Ｅ、胃蛋白酶、α－糜蛋白酶、胰酶、核酸酶消化，甲醇与氯仿除脂，０．０１ＮＨ２ＳＯ４处理等步骤提纯而得到精制完整肽聚糖（ｗｈｏｌｅｐｅｐｔｉｄｏｇｌｙｃａｎ，ＷＰＧ）。化学组分分析结果显示ＷＰＧ主要由多肽与糖组成，其氨基酸组成与菌体基本相同。扫描电镜观察发现提取的ＷＰＧ保留其原菌体细胞完整结构。
The cell wall Whole Peptidoglycan (WPG) of Bifidobacterium bifidum Bif1101 was extracted and purified as follows: (1) Treating the bacteria cells with Triton X-100. (2) Separating the insoluble residue by high speed centrifugation. (3) Digesting the insoluble residue with trypsin, α-chymotrypsin, pepsin, Pronase E, DNase and RNase. (4) Delipoiding the digested residue by successive refluxing with methanol, methanol chloroform and chloroform. (5) Treatin residue with 0.01N H 2SO 4. The purified WPG was dialyzed and lyophilized. The results of chemical analysis indicated that WPG was consisted of polysaccharide and peptide, electeonmicroscopic observation of WPG showed that WPG had the physically intact cell wall structure of the whole cells. From these results above, isolating WPG from whole cell without being subjected to physically destructive methods provide a method for extract whole WPG.
出处
《中国微生态学杂志》
CAS
CSCD
1997年第5期10-13,共4页
Chinese Journal of Microecology
关键词
双歧杆菌
肽聚糖
分离
纯化
Peptidoglycan Bififdobacterium Isolation Purification
