摘要
目的:观察川芎嗪对大鼠局灶性脑缺血再灌注损伤的影响。方法:SD大鼠随机分为6组,分别为假手术组、脑缺血再灌注模型组、尼莫地平组(0.4mg·kg^(-1))、川芎嗪组(川芎嗪60mg·kg^(-1))。采用线检法制备SD大鼠永久性大脑中动脉阻塞(permanent middle cerebral artery occlusion,pMCAO)模型。造模24h后,测定神经功能缺损评分、脑组织含水量、脑梗塞体积、血清中超氧化物歧化酶(SOD)活性、血清丙二醛(MDA)和血栓素B2(TXB2)含量,并进行组织病理学检查。结果:与模型组相比,实验组大鼠的神经行为明显改善,脑含水量和脑梗塞体积显著降低(P<0.05或0.01),血清中SOD活性升高,MDA和TXB2含量降低(P<0.05或0.01),HE染色显示组织间隙较小,病理损伤小于模型组。结论:川芎嗪对大鼠局灶性脑缺血有保护作用。
Objective：To investigate the effects of tetramethylpyrazine on focal cerebral ischemia reperfusion injury in rats.Methods：SD rats were randomly divided into 6groups,they are sham operation group,cerebral ischemia-reperfusion model group,nimodipine group（0.4mg·kg^（-1））,tetramethylpyrazine group（tetramethylpyrazine 60mg·kg^（-1））.Use line inspection method to prepare permanent middle cerebral artery occlusion（pMCAO）models.After modeling 24 h,to determine neurological deficit score,water content in brain tissue,cerebral infarct volume,serum superoxide dismutase activity（SOD）,malondialdehyde（MDA）and thromboxane B2（TXB2）content,and examine histopathological.Results：Compared with the model group,neurobehavioral experimental group improved significantly,significantly reduced brain water content and infarct volume（P0.05 or 0.01）,elevated serum SOD activity,decreased content of MDA and TXB2（P0.05 or 0.01）,HE staining showed smaller organizations gap,less than pathological damage model group.Results：Tetramethylpyrazine on focal cerebral ischemia in rats has a protective effect.
出处
《数理医药学杂志》
2016年第8期1189-1191,共3页
Journal of Mathematical Medicine
关键词
川芎嗪
局灶性脑缺血
保护作用
tetramethylpyrazine
focal cerebral ischemia
protective effect
