摘要
目的 通过对局灶性脑缺血大鼠不同再灌注时段的动态观察 ,探讨三七三醇皂苷 ( PTS)对大鼠局灶性脑缺血 /再灌注动物模型的神经行为学和脑梗死体积的保护作用。方法 采用改良的线栓法制备大脑中动脉阻塞 ( MACO) 2 h、再灌注不同时间段 ( 3h、6 h、12 h、2 4 h、4 8h、72 h、7d)的大鼠短暂局灶性脑缺血模型 ,动物随机分假手术组、生理盐水对照组、三七三醇皂苷 ( PTS)组。用 Zea L onga 5分制评分和 TTC染色法评价神经行为学和脑梗死体积。结果 神经行为学评分除 72 h组有明显改善外 ,其余各组与生理盐水对照组比较无显著性差异。脑梗死体积除再灌注 3h、6 h外 ,其余各组与生理盐水组比较差异均有显著性意义。
Objective To study the protective effects of PTS on cerebral ischemia-reperfusion injury in rats.Metheods The model of acute reperfusion injury after cerebral ischemia in rats was made bymiddle cerebral artery occlusion.6d before operation and 2h pre occlusion,the rats received intraperitoneally PTS(50 mg/kg) or normal saline injection respectively,then all the animals were allowed 2h ischemia and sacrificed after different durations of reperfusion (3h,6h,24h,48h,72h,7d).The neurological deficitgrades and the volume of the cerebral infarction were estimated by 2% TTC staining and Zea Longa's method.Results The data showed non-significant differences on the neurological deficit scores between the control and PTS-treated groups (50mg/kg) except for at 72h after reperfusion.The cerebral infarct sizes of the forebrain in the PTS-treated groups except for (3h and 6h) of reperfusion reduced significantly compared with the control groups.Conclusion PTS possesses cerebral protective effects against ischemia-reperfusion injury.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2004年第4期354-356,共3页
Journal of Apoplexy and Nervous Diseases
