摘要
目的对广州番禺地区的献血人群开展Mur抗原与抗-Mur抗体的筛查,了解该血型的分布频率和特征。方法采用96孔微量板法使用单克隆抗-Mur试剂对献血者样本进行Mur抗原检测;使用已知Mur抗原阳性试剂红细胞对献血者血浆样本进行抗-mur抗体筛查,并对筛查阳性的标本进行抗体特异性鉴定。结果在3 000份无偿献血者标本中,检出Mur抗原阳性215例,发生率约为7.17%;在15 000份献血者标本中筛选并鉴定出抗-Mur 30例,发生率为0.20%。结论本地区献血人群中存在相对较高的Mur抗原分布频率以及抗-Mur发生频率,有必要在配制不规则抗体筛选试剂细胞时增加含有Mur抗原的红细胞,防止抗-Mur抗体漏检,进一步降低临床血浆输注不良反应的风险。
Objective By screening the Mur antigen and anti-Mur among the blood donors of Panyu district of Guangzhou, analyzing the frequencies and distribution characteristics of Mur blood group in the area. Method By using monoclonal anti-Mur reagent and 96-well micro plate method, Mur antigen of blood donors were screened. By using Mur antigen positive red blood cells reagent, anti-Mur of plasma samples were screened, the screened positive samples were carried out antibody specific identification. Results In 3 000 samples of blood donors, 215 cases of Mur antigen positive samples were detected, the frequency was 7.17%. In 15 000 samples of blood donors 30 cases of anti-Mur were screened and identified, the frequency was 0.20%. Conclusion There is a relatively high frequency of Mur antigen distribution as well as the frequency of anti-Mur in blood donors in the region. It’s necessary to increase red blood cells containing Mur antigen in the preparation of irregular antibody screening reagent cells to prevent anti-Mur antibody undetected, and furtherly reduce the risk of adverse clinical plasma transfusion reaction.
出处
《临床输血与检验》
CAS
2016年第4期384-386,共3页
Journal of Clinical Transfusion and Laboratory Medicine
