摘要
目的:研究阿魏酸(ferulic acid,FA)在缺氧条件下对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)增殖、迁移和管腔样结构形成的影响。方法:原代培养人脐静脉内皮细胞,在缺氧实验条件下,细胞被分为7组,即1个对照组和6个实验组。对照组采用1%酒精处理,实验组用不同浓度(1×10^(-8)、1×10^(-7)、1×10^(-6)、1×10^(-5)、1×10^(-4)及1×10^(-3) mol/L)的阿魏酸处理。分别采用MTS法、划痕法、Matrigel法分析不同浓度阿魏酸处理对人脐静脉内皮细胞的增殖、迁移和管腔样结构形成的影响。结果:缺氧条件下,浓度为1×10^(-6)~1×10^(-4)mol/L的阿魏酸处理能明显促进HUVECs的增殖(P<0.05),以1×10^(-5) mol/L处理的效果最好(P<0.01);与对照组相比,1×10^(-6)mol/L(P<0.05)、1×10^(-5) mol/L(P<0.01)及1×10^(-4) mol/L(P<0.01)阿魏酸处理均能明显促进HUVECs横向迁移,以1×10^(-5) mol/L处理迁移的细胞数量最多;1×10^(-8)~1×10^(-4) mol/L阿魏酸处理能不同程度地促进HUVECs管腔样结构的形成,以1×10^(-5) mol/L处理形成管腔样结构的数量最多(P<0.01)。结论:阿魏酸在缺氧条件下能促进人脐静脉内皮细胞的增殖、迁移和管腔样结构形成。
Objective： To study the effects of femlic acid （FA） on the proliferation, the migration and the formation of the tube-like structure of human umbilical vein endothelial cells （HUVECs） under hypoxia. Methods： The primary HUVECs were cultured under normoxia conditions （21% 02, 5 % CO2 and 74 % N2）, and the experimental conditions ofhypoxia （1% 02, 5 % CO2 and 94 % N2） were established. Under hypoxia, the cells were divided into seven groups, one control group and six experimental groups. The con- trol group was treated with 1% ethanol, and the experimental groups were treated with different concentrations of femlic acid （1 × 10^-8, 1 × 10^-7, 1 × 10^-6, 1 × 10^-5, 1 × 10^-4 and 1 × 10^-3 mol/L）. The MTS assay was used to evaluat the effects of the proliferation activity of ferulic acid on HUVECs after 48 hours; the wound healing assay was used to detect the effects of the lateral migration ability of ferulic acid on HUVECs after 24 hours; the tube formation assay on Matrige was used to detect the effects of the tube-like structure formation ability of ferulic acid on HUVECs after 7 hours. SPSS 17.0 was used for statistical analysis. The experimental data of each group were statistically analyzed by Dunnett-t test, and P〈0.05 was statistically different. Results： Under hypoxia, the concentrations of ferulic acid between 1 × 10^-8 to 1 × 10^-5 mol/L could promote the proliferationon of HUVECs in a concentration dependent manner, and the effects of 1 × 10^-5 mol/L were the best. Compared with the control group, the differences were statistically significant （1× 10^-6 and 1 × 10^-4 mol/L, P〈0.05; 1 × 10^-5 mol/L,p〈0.01）. Compared with the control group, femlic acid could promote the migration of HUCECs, and the differences were statistically significant （1 × 10^-8 mol/L P〈0.05; 1 × 10^-5 mol/L and 1 × 10^-4 mol/L, P〈0.01）. Ferulic acid had the ability to enhance tube-like structure formation of HUVECs, and compared with the control group, the differences were statistically significant （1 × 10^-4 mol/L, P〈0. 05; 1 × 10^-5 mol/L, P〈0.01）. Conclusions： Ferulic acid can promote the proliferation, the migration and the formation of the tube-like structure of human umbilical vein vascular endothelial cells under hypoxia.
出处
《现代生物医学进展》
CAS
2016年第13期2452-2455,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金面上项目(81272118
30973752)
