摘要
背景:有研究表明封闭负压引流可以通过增加创面血流量的方式加速创面愈合,但关于其对人慢性创面中活性形式明胶酶的影响,尚缺乏相应的具体研究。目的:观察封闭负压引流技术对加速慢性创面愈合的活性形式明胶酶的影响。方法:从2013年4月到2014年1月,选取湖北医药学院附属人民医院的96例创伤患者进行临床治疗,将其分为2组,慢性创面组52例患者给予创面封闭负压引流,对照组44例患者于乳腺癌切除后第3天的创面引流液设为急性创面对照。慢性创面组将从压疮和乳癌术后皮肤坏死创面收集的渗出液标本分别成为样本A和B;从2例静脉瘀滞性溃疡创面收集的标本分别称为样本C,D;从创伤性皮肤缺损创面收集的称为样本E。结果与结论:薄层分析仪测定表明:负压治疗15 d后,慢性创面组所有样本中活性较低的活性形式的明胶酶A样本中的活性基质金属蛋白酶2,B样本中的活性基质金属蛋白酶9,C,D,E样本中的活性基质金属蛋白酶2、基质金属蛋白酶9活性较治疗前均增高(P<0.05),而活性过高的活性形式明胶酶A样本中的活性基质金属蛋白酶9,B样本中的活性基质金属蛋白酶2活性则较治疗前显著减弱(P<0.05)。治疗15 d后,慢性创面中活性基质金属蛋白酶2、活性基质金属蛋白酶9、活性形式明胶酶总活性与对照组相比均差异无显著性意义(P>0.05)。结果证实,人慢性创面中活性形式明胶酶活性的改变可能是慢性创面长期不愈的原因,封闭负压引流能调节慢性创面中活性形式明胶酶的活性。
BACKGROUND： Studies have shown that vacuum sealing drainage can accelerate wound healing through increasing the wound blood flow, but its influence on activated gelatinases in human chronic wound still lacks corresponding research. OBJECTIVE： To observe the effect of vacuum sealing drainage on the activity of gelatinase during the healing of chronic wounds. METHODS： Totally 96 trauma patients admitted at the People＇s Hospital of Hubei University of Medicine from April 2013 to January 2014 were divided into two groups： 52 patients in chronic wound group were treated with vacuum sealing drainage and 44 in control group treated with wound drainage at 3 days after removal of breast cancer. In the chronic wound group, exudates from pressure sores and skin necrosis after removal of breast cancer were collected as samples A and B; exudates from two cases of venous stasis ulcer were collected as samples C and D; exudates from traumatic skin wounds collected as sample E. RESULTS AND CONCLUSION： TLC analyzer showed that： after 15-day negative pressure therapy, activities of matrix metalloproteinase 2 in sample A with low activity of gelatinase, matrix metalloproteinase 9 in sample B, matrix metalloproteinase 2 and 9 in samples C, D, E were all increased significantly（P〈0.05）; however, the activities of matrix metalloproteinase 9 in sample A with high activity of gelatinases and matrix metalloproteinase 2 in sample B were reduced significantly after treatment（P〈0.05）. In addition, no significant difference was found in the activity of matrix metalloproteinase 2, matrix metalloproteinase 9 and activated gelatinase betweenthe chronic wound group and control group（P〈0.05）. These findings indicate that the variation of activated gelatinases activity in human chronic wounds may be the reason why the chronic wounds cannot be healed for a long time, but vacuum sealing drainage can regulate the activity of activated gelatinases in chronic wounds.
出处
《中国组织工程研究》
CAS
北大核心
2015年第25期4064-4068,共5页
Chinese Journal of Tissue Engineering Research
