摘要
目的：建立高效液相色谱法（HPLC）法测定银黄降脂袋泡茶中绿原酸和大黄素含量的方法。方法：采用DiamonsilC18柱,绿原酸和大黄素分别以乙腈-0.4%磷酸水（13∶87）和甲醇-0.1%磷酸水（80∶20）为流动相,以327nm和254nm为检测波长,流动相流速为1.0mL/min;柱温为30℃。结果：绿原酸和大黄素的线性回归方程分别为ˇy=17.955x-0.2848,相关系数r=0.9999,线性范围为0.041～0.41μg;ˇy=33.975x＋186.36,相关系数r=0.9995,线性范围0.0395～0.395μg。平均加样回收率分别100.38%（RSD为2.34%,n=9）和101.21%（RSD为2.59%,n=9）。结论：该方法简便、快速、重现性好、结果准确,可用于银黄降脂袋泡茶的质量控制。
Objective：To establish the high performance liquid chromatography（HPLC）method for the determination of chlorogenicacid and emodin in Yinhuang Jiangzhi Tea.Methods：The contents of chlorogenicacid and emodin in Yinhuang Jiangzhi Tea were determined with Diamonsil C18 column by elution of acetonitrile-0.4%phosphoric acid in water（13：87）and methanol-0.1%phosphoricacidin water（80：20）,at 327 nm and 254 nm respectively.The flow rates were 1.0mL/min,and the column temperatures were 30℃.Results：The correlation between Injection Volume and peak area of chlorogenic acid and emodin were Y=17.955X-0.2848（R=0.999 9,0.041-0.410μg）and Y=33.975X＋186.36（R=0.999 5,0.0395-0.3950μg）,respectively.The mean recovery of chlorogenic acid and emodin were 100.38%（RSD=2.34%,n=9）and 101.21%（RSD=2.59%,n=9）,respectively.Conclusions：The HPLC methods are relatively simple,accurate,and specific,and can be used for the quality control of Yinhuang Jiangzhi Tea.
出处
《海南医学院学报》
CAS
2016年第12期1200-1202,1206,共4页
Journal of Hainan Medical University
基金
上海市卫生局中药新药及院内制剂研发项目(2012Y002B)~~
