摘要
目的 建立银黄中绿原酸的含量测定方法.方法 采用高效液相色谱法测定,色谱柱 ODS C18流动相：乙腈-0.4%磷酸（13：87）;检测波长：327nm.结果 在0.03108～3.108μg范围内具有良好的线性关系（R=0.9999）,平均回收率为98.28%,RSD3.13%（n=5）.结论 本法简便,快速,重现性好.
OBJECTIVE To estabilish a HPLC method for detemination of chlorogenic acid in YingHuang capsules. METHODS HPLC method was used. Chromatographic conditions： ODS C18 column, a mixture of acetonitrile -0.4% HaPO4 （13 ： 87） as mobile phase; wavelength set at 327nm. RESULTS Linear range of chlorogenic acid was 0.03108～3. 108μg （R=0. 9999）, respectively. The average recoveries was 98.28%, RSD3.13 % （n= 5）. CONCLUSION The method was simple,rapid and good reproducibility.
出处
《海峡药学》
2006年第3期76-78,共3页
Strait Pharmaceutical Journal
关键词
绿原酸
银黄胶囊
HPLC
Chlorogenic acid
YingHuang Capsules
HPLC
