摘要
目的观察头穴丛刺法配合加味补阳还五汤灌胃对气虚血瘀型缺血性中风大鼠NSC分化的影响。方法选取健康大鼠采用气虚血瘀证候造模,成功后予Di L染料预标记。随机分为A组、B组、C组、D组、E组和F组,F组为4只,其余各组为12只。预标记48 h后行MCAO造模,制备气虚血瘀型缺血性中风大鼠模型,模型建立成功后,A组为模型组,B组采用头穴丛刺法治疗,C组采用补阳还五汤原方灌胃治疗,D组采用加味补阳还五汤灌胃治疗,E组采用头穴丛刺法配合加味补阳还五方灌胃治疗,F组为假手术组。在各时间点分别处死取材,以神经功能评定、免疫荧光双染法、激光共聚焦观测为检测指标,进行对比研究,观察各疗法对模型大鼠NSC分化的影响。结果 B组、C组、D组和E组治疗1星期后神经功能评分与A组比较,差异均具有统计学意义(P<0.01)。D组和E组治疗2星期后神经功能评分与A组比较,差异均具有统计学意义(P<0.05,P<0.01)。E组治疗1、2星期后神经功能评分与C组和D组比较,差异均具有统计学意义(P<0.01,P<0.05)。B组、C组、D组和E组治疗1、2星期后Brdu^+/Neu N^+细胞计数、Brdu^+/GFAP^+细胞计数、Dil^+/Brdu^+/Neu N^+细胞计数及Dil^+/Brdu^+/GFAP^+细胞计数与A组比较,差异均具有统计学意义(P<0.01)。D组和E组治疗1、2星期后Brdu^+/Neu N^+细胞计数、Brdu^+/GFAP^+细胞计数、Dil^+/Brdu^+/Neu N^+细胞计数及Dil^+/Brdu^+/GFAP^+细胞计数与B组和C组比较,差异均具有统计学意义(P<0.01)。E组治疗1、2星期后Brdu^+/Neu N^+细胞计数、Brdu^+/GFAP^+细胞计数、Dil^+/Brdu^+/Neu N^+细胞计数及Dil^+/Brdu^+/GFAP^+细胞计数与D组比较,差异均具有统计学意义(P<0.01)。结论各种治疗方法对模型大鼠神经干细胞的分化均具有一定的促进作用,其中以头穴丛刺法配合加味补阳还五汤灌胃效果最佳。
Objective To investigate the effect of cluster needling at scalp points plus gavage of modified Buyang Huanwu decoction on NSC differentiation in rats with ischemic stroke of qi deficiency and blood stasis type. Methods A model of qi deficiency and blood stasis syndrome was made using healthy rats. Dil dye was given for pre-labelling after the success of model making. The rats were randomized into groups A, B, C, D, E and F. Group F consisted of 4 rats and each of the other groups, of 12 rats. A rat model of ischemic stroke of qi deficiency and blood stasis type was made by MCAO at 48 hrs after pre-labelling. After the success of model making, group A was the model group, group B was treated by cluster needling at scalp points, group C was treated by an oral gavage of Buyang Huanwu decoction, group D was treated by an oral gavage of modified Buyang Huanwu decoction, group E was treated by cluster needling at scalp points plus an oral gavage of modified Buyang Huanwu decoction and group F was the sham operation group. The rats were sacrificed at various time points respectively and the materials were taken. By nerve function assessment, double immunofluorescence staining and laser scanning confocal microscopy, a comparative study was conducted to investigate the effects of different treatments on NSC differentiation. Results There was a statistically significant difference in the nerve function score between group B, C, D, E or F and group A after one week of treatment（p〈0.01）, between group B or E and group A after two weeks of treatment（p〈0.05,p〈0.01） and between group E and group C or D after one and two weeks of treatment（p〈0.01,p〈0.05）. After one and two weeks of treatment, there were statistically significant differences in Brdu﹢/Neu N﹢ cell count, Brdu﹢/GFAP﹢ cell count, Dil﹢/Brdu﹢Neu N﹢ cell count and Dil﹢/Brdu﹢/GFAP﹢ cell count between group B, C, D or E and group A（p〈0.01）, between group D or E and group B or C（p〈0.01） and between groups E and D（p〈0.01）. Conclusions Various treatments have a promoting effect on nerve stem cell differentiation in the rats. Of various treatments, cluster needling at scalp points plus gavage of modified Buyang Huanwu decoction has the best therapeutic effect.
出处
《上海针灸杂志》
2016年第3期355-358,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
黑龙江省教育厅科学技术研究项目(12531659)
关键词
针刺疗法
中风
气虚血瘀
补阳还五汤
针药并用
丛刺
大鼠
头针
Acupuncture therapy
Stroke
Qi deficiency and blood stasis
Buyang Huanwu decoction
Combined acupuncture and medicine
Cluster needling
Rats
Scalp acupuncturee
