摘要
目的建立同时测定四妙勇安颗粒中哈巴苷、绿原酸、阿魏酸、甘草苷、哈巴俄苷和甘草酸含量的高效液相色谱方法。方法采用Kromasil-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相:乙腈-0.4%磷酸水,梯度洗脱,流速:1.0 m L·min^(-1),检测波长:210 nm(哈巴苷)、324 nm(绿原酸、阿魏酸、甘草苷)、278 nm(哈巴俄苷、甘草酸),柱温30℃。结果哈巴苷、绿原酸、阿魏酸、甘草苷、哈巴俄苷和甘草酸分别在进样量0.148~2.960μg(r=0.9997)、0.423~8.460μg(r=0.9996)、0.024~0.480μg(r=0.9998)、0.151~3.020μg(r=0.9997)、0.035~0.700μg(r=0.9995)、0.197~3.940μg(r=0.9996)内线性关系良好,加样回收率分别为97.1%、97.3%、101.3%、96.7%、99.3%、96.5%。结论本文建立的方法符合方法学验证要求,可用于四妙勇安颗粒中6个指标性成分的同时测定。
Objective To develop an HPLC method for the simultaneous determination of 6 active components （harpagide, chlorogenic acid, ferulic acid, liquiritin, harpagoside and glycyrrhizic acid） in Simiao Yong＇an granules. Methods The sample was separated on an Kromasil-C18 column （4.6 mm×250 mm,5μm） with a mobile phase consisting of acetonitrile （A）-0.4% phosphoric acid solution （B） with gradient elution. The flow rate was 1.0 mL · min - 1. The detection wavelength was 210 nm for harpagide; 324 nm for chlorogenic acid, ferulic acid and liquiritin; 278 nm for harpagoside and glycyrrhizic acid, and the column temperature was 30 ℃ . Results The method had a good linearity at 0.148 - 2.960 μg （r = 0.9997） for harpagide, 0.423 - 8.460 μg （r=0.9996） for chlorogenic acid, 0.024 - 0.480 μg （r = 0.9998） for ferulic acid, 0.151 - 3.020 μg （r = 0.9997） for liquiritin, 0.035 - 0.700 μg （r = 0.9995） for harpagoside, and 0.197 - 3.940 μg （r= 0.9996） for glycyrrhizic acid. The recoveries were 97.1%, 97.3%, 101.3%, 96.7%, 99.3% and 96.5%. Conclusion This method is con- sistent with the validation requirements, which can simultaneously determine the above 6 active components in Simiao Yong＇an granules.
出处
《中南药学》
CAS
2016年第1期69-73,共5页
Central South Pharmacy
