摘要
以洋葱T型细胞质雄性不育系8-43A、保持系8-43B和恢复系8-43-57C为试材,采用萤火虫荧光素酶检测技术,分析花蕾不同发育时期三磷酸腺苷(ATP)的含量变化,研究洋葱T型细胞质雄性不育与花蕾ATP含量的关系。结果表明:在整个花药发育过程中的各个阶段,保持系和恢复系的花蕾之间ATP含量均无显著差异,不育系花蕾ATP含量均极显著低于保持系和恢复系,尤其在花粉粒成熟早期,仅为保持系和恢复系的1%;花粉粒成熟早期,不育系花蕾ATP含量下降到不足四分体时期的1/10,说明花药发育通过单核期后耗费了组织内大量的ATP,更重要的是ATP的产生可能出现了障碍;洋葱T型细胞质雄性不育系花蕾ATP含量极显著低于各时期的可育株,可能是导致其雄性不育的原因。
Taking onion T-type cytoplasmic male sterile line ‘8-43A＇,maintainer line ‘8-43B＇and restorer line ‘8-43-57C ＇as material,using firefly luciferase enzyme testing technology,this paper analyzed the changes in ATP contents of buds at different growing stages,and studied the relationships between ATP contents of buds and T-type cytoplasmic male sterility in onion.The results showed that there were no significant differences between the ATP contents of maintainer and restorer lines throughout the entire development process of bud.While,the ATP content of CMS bud was very significantly lower than that of the maintainer and restorer lines,especially at early grain stage,which only took 1% of that in maintainer and restorer lines.ATP content of the CMS line at early grain stage decreased to less than 1/10 of those at tetrad stage,illustrating a large quantity of ATP was consumed after mononuclear stage.And an even important thing was there might be barriers in ATP generation.The ATP content of T-type CMS in onion was very significantly lower than that of the fertile lines might be the reason for male sterility.
出处
《中国蔬菜》
北大核心
2016年第2期32-35,共4页
China Vegetables
基金
国家自然科学基金青年基金项目(31201638)
