摘要
目的:探讨Hedgehog信号通路在调控骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖、成骨细胞(osteoblast,OB)分化和成骨能力、骨形成中的生物学调控作用以及运动训练的影响。方法:48只4周龄C57BL/6雄性小鼠,适应性喂养1周后随机分为对照组(C组)、游泳组(S组)和下坡跑组(D组),每组16只。S组和D组分别进行游泳(45 min/d,5 d/周,共8周)和下坡跑(坡度-9°,45 min/d,5 d/周,共8周)训练。最后1次训练结束后,断颈椎处死小鼠,利用RT-PCR法检测骨中相关细胞因子mRNA表达;利用SRB染色检测BMSCs增殖能力;利用ALP染色和Von Kossa染色检测OB分化和矿化能力;利用Hologic Discovery A骨密度仪检测骨密度。结果:与C组相比,S组小鼠骨中Ihh(P<0.05)和Shh(P<0.05)mRNA表达均出现显著上调,第2 d(P<0.05)和第4 d(P<0.01)的BMSCs数量、OB的ALP活性(P<0.01)均出现显著升高;与C组相比,D组小鼠骨中Ihh(P<0.01)、Shh(P<0.01)、Ptch(P<0.01)和Smo(P<0.01)mRNA表达均出现显著上调,第2 d(P<0.01)和第4 d(P<0.01)的BMSCs数量、OB的ALP活性(P<0.01)、OB矿化能力(P<0.01)和BMD(P<0.01)均出现显著升高;与S组相比,D组小鼠骨中Ihh(P<0.05)、Shh(P<0.05)、Ptch(P<0.05)和Smo(P<0.05)mRNA表达均显著上调,第2 d(P<0.01)和第4 d(P<0.01)的BMSCs数量、OB的ALP活性(P<0.05)、OB矿化能力(P<0.05)、BMD(P<0.01)均出现显著升高。结论:运动干预可通过Hedgehog信号通路调控BMSCs增殖和OB分化及成骨能力从而促进骨形成,且下坡跑作用效果优于游泳。
Objective ： This study explored the effect of Hedgehog signaling pathway on the regulation of proliferation of bone marrow mesenchymal stem cells, osteoblast （OB） differentiation, osteogenic capability and bone forma- tion, and also detected the effect of exercise on this regulation. Methods ： After one week adaptation, 48 male 4- week old C57BL/6 mice were randomly divided into three groups （n = 16） ： control group （group C） , swimming group （group S） and downhill running group （group D）. Group S and group D underwent swimming （45min/d, 5d/week, 8 weeks in total） and downhill run （grade：-9, 45rain/d, 5d/week, 8 weeks in total）. After all the training, the mice were killed. The mRNA expressions of cell cytokines were tested by TR-PCR, proliferation of bone marrow mesenchymal stem cells （BMSCs） was tested after SRB staining, the differentiation and mineraliza- tion capabilities of OB were tested after ALP and Von Kossa staining, and bone mineral density was tested by Ho-logic Discovery A. Results ： Compared with group C, the mRNA expressions of bone Ihh and Shh in group S were increased （ Ps 〈 0. 05）, the number of BMSCs and activity of OB ALP in the second and fourth days in group S were increased（ Ps 〈0.05）. Compared with group C, the mRNA expressions of bone Ihh, Shh, Ptch and Smo in group D were increased （Ps 〈 0.01 ）, the number of BMSCs, activity of OB ALP, bone mineralization capability and BMD of group D in the second and fourth day were significantly increased （Ps 〈 0. 01 ）. Compared with group S, the mRNA expressions of bone Ihh, Shh, Ptch and Smo in group D were increased （Ps 〈0. 05 ）, the number of BMSCs, activity of OB ALP, bone mineralization capability and BMD of group 1） in the second and fourth day were significantly increased （Ps 〈 0. 05 ）. Conclusion： Exercise intervention promotes bone formation by promo- ting Hedgehog signal way to regulate BMSCs proliferation and OB differentiation and osteogenic ability, and the effect of downhill run is better than swimming.
出处
《北京体育大学学报》
CSSCI
北大核心
2015年第11期59-64,共6页
Journal of Beijing Sport University
基金
中国博士后面上项目(编号:2014M551356)
<青少年POWER工程协同创新中心>项目(编号:44801400)
