摘要
目的探讨力达霉素经肝动脉介入治疗和静脉化疗两种给药途径对VX2兔肝癌的影响。方法开腹方法将VX2瘤组织块分别植入12只新西兰大白兔的肝左叶,建立VX2兔肝癌模型,B超检测肿瘤生长情况后随机分为两组:A组为力达霉素肝动脉介入组,B组为力达霉素静脉化疗组(6只/组)。A组在数字减影血管造影(DSA)引导下经肝固有动脉注入力达霉素(1 ml,0.05 mg/kg),B组通过耳缘静脉注入力达霉素(1 ml,0.05 mg/kg),给药10 d后活杀两组动物,取肝肿瘤组织4%多聚甲醛固定,免疫组化方法测定CD34、增殖细胞核抗原(PCNA)蛋白的表达;应用Cobas 8000检测血清生化丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平;计算并比较两种不同给药方式的肿瘤生长抑制效果。结果给药10 d后两组肿瘤均有增大,但与B组相比,A组肿瘤生长率明显降低(P<0.05);免疫组化结果显示,介入途径给药更能下调CD34、PCNA表达水平。结论在治疗兔肝癌VX2中,力达霉素经肝动脉介入给药治疗效果优于静脉化疗给药。
Objective To investigate the difference of effect between interventional treatments and intravenous therapy of lidamycin on VX2 rabbit liver cancer.Methods VX2 Carcinoma cells were surgically implanted into the left liver lobe of 12 New Zealand white rabbits to establish the VX2 rabbit liver tumor model.Tumor size was detected by type-B ultrasonic diagnostic instrument.The rabbits were randomly divided into two groups of six,respectively treated with the hepatic inter-ventional administration of lidamycin （LDM）（1 ml,0.05 mg/kg）under the guidance of digital subtraction angiography （DSA）（group A）and with the auricular intravenous administration of LDMat the same dose （group B）.All the rabbits were sacrificed and anatomized on day 10 after treatment,whose liver tumor was fixed with 4% paraformaldehyde solution and embedded in paraffin.Proliferating cell nuclear antigen （PCNA）and CD34 expression in the sample sections of tumor tissue were assessed through immunohistochemical staining.The levels of alanine transaminase （ALT）and aspartate trans-aminase（AST）were detected by Cobas 8000.Finally,the inhibition of VX2 tumor was evaluated.Results The VX2 tumor volumes were all increased at 10 day after LDMtreatment.However,the tumors in group A were smaller than those of group B （P ＆lt;0.05）.The results of immunohistochemistry showed that the intervention therapy of LDM could further lower the expression of CD34 and PCNA compared to group B.Conclusion Hepatic interventional administration of LDM＆amp;nbsp;under the guidance of DSA produces a better effect on attenuating the tumor growth than the intravenous administration of LDM.
出处
《军事医学》
CAS
CSCD
北大核心
2015年第11期850-854,共5页
Military Medical Sciences
基金
国家自然科学基金青年资助项目(81201765)
河南省高等学校重点科研资助项目(15A320063)
关键词
力达霉素
介入治疗
VX2
肝肿瘤
免疫组化
lidamycin
interventional treatments
VX2 lirer neoplasm
immunohistochemistry
