摘要
目的观察MK571对脓毒症大鼠急性肺损伤的影响。方法将45只雄性SD大鼠随机分为假手术组、脓毒症组和MK571干预组,每组15只。脓毒症组和MK571干预组采用盲肠结扎穿刺法制作脓毒症模型。MK571干预组术前30 min腹腔注射MK571 25 mg/kg。术后12 h和24 h采静脉血分别检测血清白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平。术后24 h行支气管肺泡灌洗,留取灌洗液行白细胞计数,并留取肺组织做病理学观察评估肺组织损伤程度。结果脓毒症组大鼠术后血清IL-1β和TNF-α水平及支气管肺泡灌洗液中白细胞计数均显著高于假手术组(P均<0.05),肺组织损伤明显。MK571干预组大鼠血清IL-1β和TNF-α水平及支气管肺泡灌洗液中白细胞计数均明显低于脓毒症组(P均<0.05),肺组织损伤明显减轻。结论 MK571可有效减轻脓毒症大鼠急性肺损伤。
Objective It is to explore the effect of MK571 on sepsis-induced acute lung injury in rats. Methods Forty-five SD rats were randomly divided into three groups： sham-operated group, sepsis group, and sepsis plus MRP4 inhibitor treatment group（ intervention group） , with 15 rats in each group. Sepsis was induced by cecal ligation and puncture in sepsis group and intervention group. MK57t （25 mg/kg） was administrated by intraperitoneal injection 30 minutes before induction of sepsis in intervention group. Serum interlukin - 1β （ IL - 1β） and tumor necrosis factor alpha （TNF - α） levels were measured by enzyme-linked immunosorbent assay at 12 hours and 24 hours after surgery respectively. At 24 hours after surgery, the lungs were collected for histopathological examination, and bronchoalveolar lavage fluid was harvested for white blood cellcounting. Results Compared with sham group, serum IL - 6 and TNF -α levels were significantly higher in sepsis group. White blood cell count in bronchoalveolar lavage fluid was elevated and lung injury was increased in sepsis group compared to sham group. Importantly, MK571 treatment significantly decreased serum IL -6 and TNF α levels, reduced white blood cell count in bronchoalveolar lavage fluid, and improved lung injury. Conclusion MK571 can effectively relieve acute lung injury in septic rats.
出处
《现代中西医结合杂志》
CAS
2016年第1期1-3,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金资助项目(81301620)
