摘要
目的 比较12C重离子与γ射线对人外周血淋巴细胞Peng-EBV增殖抑制率、细胞周期和凋亡率的影响.方法 对Peng-EBV细胞分别进行2.0 Gy的12C重离子和γ射线照射,通过MTS法检测细胞增殖变化,流式细胞术检测细胞凋亡率和周期分布.结果 2.0Gy重离子射线和γ射线作用后,Peng-EBV细胞的增殖抑制率均显著升高.与对照组相比,2.0 Gy射线照射后24、48、72 h细胞增殖抑制率差异有统计学意义（重离子射线：t=10.53、4.11、37.30,P＜0.05;γ射线：t=7.18、8.26、26.11,P＜0.05）.12C重离子对细胞的增殖抑制率在照射后72 h内呈时间依赖性升高,而γ射线则在照射后48h时增殖抑制率最高,而后降低.重离子射线照射后12h和24h,细胞凋亡率分别较对照组增加了7.92和13.41倍（t=7.09、4.13,P＜0.05）;而γ射线照射后12 h和24 h,细胞凋亡率分别为对照组的2.43和5.64倍（t=8.98、20.52,P＜0.05）.重离子射线照射后12h和24 h,细胞G2/M期阻滞程度分别较对照组增加5.11和11.77倍（t=12.06、100.08,P＜0.05）;而γ射线照射后12 h和24 h,细胞G2/M期阻滞程度分别为对照组的5.82和7.68倍（t=19.35、10.30,P＜0.05）.结论 12C重离子对人外周血淋巴细胞造成的生物学效应远高于γ射线.
Objective To compare the effects of carbon heavy ion and γ ray on cell survival, cell cycle and cell apoptosis.Methods Peng-EBV cells were irradiated by carbon heavy ion and X-ray.MTS was used to detect the cell survival.Cell cycle and apoptosis were analyzed by flow cytometry.Results The proliferation inhibition rate of Peng-EBV cell was significantly increased after 2.0 Gy heavy ion rays and γ rays.Compared with the control group, the cell proliferation inhibition rate difference was statistically significant after 2.0 Gy rays at 24 h, 48 h, 72 h （heavy rays： t=10.53, 4.11, 37.30, all P〈0.05;γ rays： t=7.18, 8.26, 26.11, all P〈0.05）.The cell proliferation inhibition rate showed a time-dependent increase, and reached its peak at 72 h after heavy ion rays, while the cell proliferation inhibition rate after γ ray reached its peak at 48 h, and then decreased.Compared with the control group, the apoptosis rate of Peng-EBV cell increased 7.92 and 13.41 times respectively at 12 h and 24 h after 2.0 Gy heavy ion rays （t=7.09, 4.13, both P〈0.05）, while it was 2.43 and 5.64 times after 2.0 Gy γ rays （t=8.98, 20.52, P〈 0.05）.Compared with the control group, the G2/M phase arrest increased 5.11 and 11.77 times respectively at 12 h and 24 h after 2.0 Gy heavy ion rays （t=12.06, 100.08, both P〈0.05）, however, it was 5.82 and 7.68 times after 2.0 Gy γ rays（t=19.35, 10.30, both P〈0.05）.Conclusion 12C heavy ion irradiation shows more stronger biological effects on human peripheral blood lymphocyte than γ ray.
出处
《国际放射医学核医学杂志》
2015年第5期385-388,共4页
International Journal of Radiation Medicine and Nuclear Medicine
关键词
重离子
Γ射线
淋巴细胞
辐射
生物效应
Heavy ions
Gamma rays
Lymphocytes
Radiation
Biological effect
