摘要
目的检测血清和糖皮质激素诱导的激酶1(SGK1)在川崎病(KD)患者CD4^+T细胞中的表达,探讨SGK1与维甲酸相关孤儿核受体C(RORC)及白细胞介素17A的相关性。方法收集KD患者30例,正常同年龄对照30例,同年龄感染性疾病(ID)患者25例。利用流式细胞术检测Th17细胞占CD4^+T细胞的比例;实时定量PCR检测CD4^+T细胞中SGK1和RORC mRNA的表达;ELISA检测血清IL-17A和IL-6水平。结果与健康儿童和ID患者相比,KD患者中Th17细胞比例明显升高。与ID患者和健康儿童相比,KD患者血清中IL-17A和IL-6水平明显升高,SGK1和RORC mRNA水平升高。SGK1在KD冠状动脉损害(CAL)组的表达高于冠状动脉正常(CAN)组。静脉内注射免疫球蛋白(IVIG)治疗后,SGK1的表达明显下降;其中,CAL组下降高于CAN组。KD患者中,SGK1 mRNA的表达与RORC及血清IL-17A水平呈正相关,而与血清IL-6水平不相关。结论 KD患者CD4^+T细胞中SGK1 mRNA表达升高,与RORC和IL-17A正相关。
Objective To investigate the expression of serum- and glucocorticoid-inducible kinase 1 （SGK1） in Kawasaki disease （KD） and explore the correlations between SGK1 and Th17 cell-related cytokines [ retinoic-acid-related orphan nuclear receptor C （ RORC）, intedeukin 17A （ IL-lTA）, IL-6] in KD. Methods Thirty patients with KD [ （2.8 ±1.4） years old], 30 healthy volunteers [ （2.6 ± 1.6） years oldl and 25 patients with infectious disease （ID） [ （2.2 ±1.5 ） years old] were recruited. The percentage of Th17 cells in CD4 ＋ T cells was analyzed using flow cytometry. The mRNA levels of RORC and SGK1 in CD4＋ T cells were detected using real-time quantitative PCR. The serum levels of IL-17A and IL-6 were analyzed by ELISA. Results Compared with healthy volunteers and patients with ID, the percentage of Th17 cells in CD4＋ T cells significantly increased in KD [ （3.57 ±0.62）% vs （0.51±0.07）% or （1.72±0.36）%]. The serum levels of IL-17A and IL-6 in KD were much higher than those in healthy volunteers and patients with ID. The mRNA levels of RORC and SGK1 in KD were remarkably elevated compared with healthy volunteers and patients with ID. The level of SGK1 in coronary artery lesion （CAL） group of KD was significantly higher than that in KD patients with normal coronary artery （CAN）. SGK1 was reduced in KD after intravenous immunoglobulin （IVIG） treatment. What＇s more, the decrease of SGK1 was more obviously in the CAL group than in the CAN group. In addition, SGK1 expression in KD was positively correlated with RORC and IL-17A, but not with IL-6. Conclusion SGK1 was up-regulated in CD4 ＋ T cells and was positively correlated with RORC and IL-17A in the patients with KD.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第10期1378-1382,共5页
Chinese Journal of Cellular and Molecular Immunology
