摘要
为了探明朱砂叶螨刺吸胁迫对玉米体内化学防御的系统诱导效应,以玉米抗螨性自交系‘H1014168’和感螨性自交系‘H1014591’幼苗为材料,在叶螨刺吸第1叶7 d后,测定了未被刺吸部位(第2叶和根系内)的丁布、总酚、胰蛋白酶抑制剂(TI)和胰凝乳蛋白酶抑制剂(CI)含量.结果表明:H1014168第2叶和根内的丁布、总酚、TI和CI含量均显著高于健康植株;H1014591第2叶内4种化学防御物质含量显著高于健康植株,根内TI含量显著高于健康植株,丁布和CI含量与健康植株差异不显著,总酚含量显著低于健康植株.朱砂叶螨刺吸胁迫对玉米体内的丁布、总酚、胰蛋白酶抑制剂和胰凝乳蛋白酶抑制剂的诱导具有系统性,对玉米抗螨性自交系的系统诱导效应强于感螨性自交系.
In the present study,we investigated the systematically induced production of defenserelated compounds,including DIMBOA,total phenol,trypsin inhibitors（ TI） and chymotrypsin inhibitor（ CI）,by Tetranychus cinnabarinus infestation in Zea mays. The first leaves of two corn inbred line seedlings,the mite-tolerant line ‘H1014168＇and the mite-sensitive line ‘H1014591＇,were sucked by T. cinnabarinus adult female for seven days,and then the contents of DIMBOA,total phenol,TI and CI were measured in the second leaf and in the roots,respectively. Results showed that as compared to the unsucked control,all contents of DIMBOA,total phenol,TI and CI induced by T. cinnabarinus sucking were significantly higher in the second leaf of both inbred lines as well as in the roots of the mite-tolerant ‘H1014168＇. However,in the roots of ‘H1014591＇,these defense compounds had different trends,where there was a higher induction of TI and a lower level of total phenol than that of the healthy control,while had almost no difference in DIMBOA and CI. These findings suggested that the infestation of T. cinnabarinus could systematically induce accumulation of defense-related compounds,and this effect was stronger in the mite-tolerant inbred line than in the mite-sensitive inbred line.
出处
《应用生态学报》
CAS
CSCD
北大核心
2015年第9期2824-2828,共5页
Chinese Journal of Applied Ecology
基金
国家转基因生物新品种培育科技重大专项(2011ZX08003-001)资助
