摘要
目的:本文分析Notch1信号通路在奥沙利铂诱导胃癌细胞SGC-7901凋亡中的参与作用,为相关临床治疗提供参考。方法:CCK8法分析奥沙利铂诱导胃癌细胞SGC-7901的细胞活力,Western Blotting分析Notch信号通路关键蛋白Notch1/2及细胞凋亡相关蛋白Caspase9在此过程中的表达情况。结果:奥沙利铂明显降低了胃癌细胞SGC-7901的细胞活力,上调Notch1/2及Caspase9蛋白的表达,而Notch通路抑制剂L685458可以明显恢复上述蛋白的表达异常。结论:奥沙利铂诱导明显促进胃癌细胞SGC-7901的细胞凋亡过程,且激活的Notch1/2信号通路参与了此病理过程。
Objective： To explore the role of Notchl/2 signaling pathway in the apoptosis process of SGC-7901 induced by oxaliplatin. Methods： The cell viability was detected by CCK8 and the expression of Notch1/2 and Caspase9 was detected by Western Blotting before and after treatment of oxaliplatin. Results： Oxaliplatin medication decreased the viability of SCG-7901 and increased the Notchl/2 as well as Caspase9 expression. Notch signaling pathway inhibitor L685458 normalized those ab- normalities greatly. Conclusion： Oxaliplatin promotes SGC-7901 apoptosis by activating Notch signaling pathway and up-regu- lating Caspase9 protein.
出处
《海南医学院学报》
CAS
2015年第8期1027-1029,1032,共4页
Journal of Hainan Medical University
基金
佛山市医学科研基金项目(2015325)~~
