摘要
目的建立从隔山消中分离制备4种苯乙酮对照品的方法。方法采用溶剂提取,应用色谱技术分离纯化,根据化合物理化性质和光谱数据鉴定结构,用薄层色谱(TLC)法、高效液相色谱(HPLC)法进行纯度检查。结果制备得到的对照品经薄层色谱检查无杂质,纯度均在98%以上,符合中药化学对照品含量测定要求。结论制备所得的对照品可用于隔山消及其制剂的质量控制。
Objective To establisb a metbod for tbe isolation and preparation of 4 reference substances of acetopbenone from Cy-nanchum auriculatun. Methods Cbromatograpbic tecbniques were employed for isolation and purification of tbe constituents and tbeir structures were determined by spectral analysis and cbemical evidence. Tbe purity was analyzed by TLC and HPLC. Results Tbe purity levels of tbese compounds were all over 98%,wbicb was in auordance witb tbd content determination requirement of tbe TCM cbemical reference substance. Conclusion Tbese compounds can be used as a reference substance in tbe quality control and tbe preparations of Cynanchum auriculatun.
出处
《中国药业》
CAS
2015年第16期12-13,共2页
China Pharmaceuticals
基金
国家科技支撑计划项目
项目编号:2013BAI11B01
贵州省科技厅资助项目
项目编号:[2013]4001
[2013]5062
关键词
隔山消
苯乙酮
对照品
Cynanchum auriculatun
acetopbenone
reference substance
