摘要
目的:评价白首乌C-21甾体苷(CGB)体外抑制大鼠胶质瘤C6细胞生长的作用。方法:C6细胞在CGB处理24、48、72 h后,以MTT法检测细胞活性;AnnexinV/PI双染或用PI单染后,流式细胞术检测细胞凋亡及细胞周期。结果:CGB 30、60、120 mg/L显著抑制C6细胞活性(P<0.001),呈浓度、时间依赖性;CGB 60、120 mg/L显著提高细胞凋亡率(P<0.001),并能明显阻滞细胞周期,增加G0/G1期细胞比例(P<0.05),降低S期细胞比例(P<0.01)。结论:CGB能显著抑制体外培养的大鼠胶质瘤细胞的生长,并能诱导细胞凋亡,阻滞细胞周期。
Objective:To evaluate the inhibitive effect of C-21 steroidal glycosides from the root of Cynanchum auriculatum(CGB) on rat glioma C6 cells.Methods:C6 cells were treated with CGB for 24,48,72 h at concentration of 30,60,120 mg/L,respectively.MTT assay was used for evaluating cell viability;fluorescence-activated cell sorting analysis after Annexin V/propidium iodide staining or single propidium iodide staining was used to test cell apoptosis and cell cycle.Results:CGB at 30,60,120 mg/L concentration-dependently decreased C6 cell viability(P<0.001).CGB at 60 and 120 mg/L induced C6 cell apoptosis and cell cycle arrest.The fraction of G0/G1 cells was increased(P<0.05) and that of S phase cells was decreased(P<0.01).Conclusion:CGB can inhibit the growth of rat glioma C6 cells,and induce apoptosis and G0/G1 cell cycle arrest.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2011年第4期402-407,共6页
Journal of Zhejiang University(Medical Sciences)
基金
浙江中医药大学重点资助项目(09ZZ-2)
