摘要
目的观察射频消融联合瘤周注射胞嘧啶鸟嘌呤寡脱氧核苷酸（CpGODN）对BALB／c小鼠皮下种植性乳腺癌的治疗作用及对荷瘤鼠免疫功能的影响。方法32只BALB／c小鼠双侧胁肋部皮下接种乳腺癌细胞EMT-6，建立乳腺癌动物模型，随机分为4组，每组8只，分别给予左侧皮下肿瘤射频消融＋瘤周注射CpGODN、射频消融、瘤周注射CpGODN和瘤周注射生理盐水4种处理。定期测量治疗侧及非治疗侧肿瘤大小，尾静脉取血法收集各组小鼠外周血，酶联免疫吸附试验（ELISA）法检测血清白细胞介素（IL）-12、IL-10、IL-2及干扰素（IFN）-γ含量。当非治疗侧肿瘤直径达到18mm时手术切除肿瘤，并记录为非治疗侧观察终点，当治疗侧肿瘤直径达到18min时或出现恶病质时处死小鼠，记录生存时间。结果射频消融＋瘤周注射CpGODN组和射频消融组治疗侧肿瘤体积体积显著小于CpGODN组和对照组（P〈0．05）。射频消融＋瘤周注射CpGODN组未治疗侧的肿瘤体积在第21天后明显小于其他各组（P〈0．05）。射频消融＋瘤周注射CpGODN组中位生存时间为92d，射频消融组为84d，瘤周注射CpGODN组为64d，对照组为60d。射频消融＋瘤周注射CpGODN组小鼠外周血中IL—12浓度为312．40ng／L，IL-10浓度为216．34ng／L，IL-2浓度为75．33ng／L，IFN-γ浓度为69．32ng／L，显著高于其他3组（P〈0．05）。结论射频消融联合瘤周注射CpGODN能激活小鼠体内的抗肿瘤免疫效应，起到抑制肿瘤生长作用。
Objective To investigate the efficacy of microwave ablation combined with peritumoral injected cytosine phosphate guanine oligodeoxyribonucleotide （CpG ODN） in inducing immunityresponse against mouse breast cancer. Methods Bilateral breast cancer model was established by subcutaneous injec- tion EMT - 6 ceils underneath both sides of the BALB/c mouse rib area. According the therapy method to the left side tumor, there were 32 mice were divided randomly into four groups： combination therapy group （mi- crowave ablation combined with peritumoral injected CpG ODN）, ablation therapy group, CpG ODN therapy group, and control group. Bilateral tumor volume and survive time were observed. Concentration of interleu- kin （IL） -12, IL- 10, IL- 2 and interferon （IFN） -γ in serum were detected by enzyme linked immu- nosorbent assay （ELISA） method. When the non- treatment side tumor reaches 18 mm in diameter, the tumor resection is carried on, and recorded as endpoint of this side. When the treatment side tumor reaches 18 mm in diameter or cachexia takes place, the mice will be kilIed and the survival time will be recorded. Results In the bilateral tumor model, the left tumor volumes in combination therapygroup and ablation ther- apy group were smaller than those in CpG ODN therapy group and control group, right tumor volumes in com- bination therapygroup were smaller than those in other group after 21 days. The median survive time in com- bination therapy group, ablation therapy group, CpG ODN therapy group, and control group were 92, 84, 64, 60 days respectively. The serum concentrations of IL - 2, IL - 10, IL - 12, IFN -γ in combination ther- apy group were higher than those in other groups. Conclusion Microwave ablation combined with peritu- moralinjection of CpG ODN can enhance the host antitumor iinmunityeffect and inhibit tumor growth.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第8期1869-1871,共3页
Chinese Journal of Experimental Surgery
基金
广东省科技计划资助项目（20128061700065）
广东省医学科研基金资助项目（B2012191）
关键词
射频消融
胞嘧啶鸟嘌呤寡脱氧核苷酸
乳腺癌
免疫治疗
Ablation
Cytosine phosphate guanine oligodeoxyribonucleotide
Breast cancer
Immunotherapy
