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SLE病人T淋巴细胞亚群功能紊乱的研究 被引量:7

THE STUDY OF ABNORMAL FUNCTION IN T CELLS OF SYSTEMIC LUPUS ERYTHEMATOSUS
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摘要 目的:对系统性红斑狼疮(systemic lupus erythematosus,SLE)病人外周血T淋巴细胞表型CD 3+CD4+CD 8+进行检测,了解T细胞亚群在SLE的变化,同时测定主要由T辅助细胞(Th细胞)分泌的细胞因子:血清白细胞介素-2(Interleukin-2,IL-2)和白细胞介素-6(Interleukin-6,IL-6)的水平,以探讨细胞因子的异常表达在SLE病人疾病中的作用。方法:用流式细胞仪分析SLE病人和健康对照组外周血T淋巴细胞表面抗原;采用双抗体夹心酶联免疫吸附试验(ELISA)检测SLE病人和健康对照组血清中IL-2和IL-6水平。结果:SLE病人CD 3+CD 4+细胞较正常对照组明显降低(P<0.01),CD 3+CD 8+细胞较正常对照组增加(P<0.05);SLE病人组CD 4+T/CD 8+T<1,CD 4+T细胞与CD 8+T细胞计数明显倒置,正常对照组CD 4+T/CD 8+T>1。SLE病人血清IL-2水平较正常对照组明显降低(P<0.01),血清IL-6水平较正常对照组增高(P<0.05)。结论:T细胞亚群水平及Th1/Th2平衡向Th2细胞占优势漂移对于SLE的发病及病情活动度的衡量有着重要的意义。 Objective: To detect the phenotype in peripheral blood T lymphocytes in patients with systemic lupus erythematosus( SLE),and investigate the change in SLE T-cell subsets. To detect serum levels of Interleukin-2( IL-2) and Interleukin-6( IL-6),mainly secreted by T helper cell,and to explore the change in Th 1 and Th 2 cells with abnormal regulation of cellular immunity during the activation expression in SLE patients and the role of abnormal expression cytokines. Methods: Peripheral blood T lymphocyte surface antigen in SLE patients and healthy control group were measured by flow cytometry; serum levels of IL-2 and IL-6 in SLE patients and healthy control group were detected by double-antibody sandwich enzyme-linked immuno sorbent assay( ELISA). Results: Significantly decreased CD 3+CD 4+cell was found in SLE patients as compared to that in healthy controls( P 〈0. 01). Increased CD 3+CD 8+cell was found in SLE patients as compared to that in healthy controls( P 〈0. 05). CD 4+T / CD 8+T 1 in SLE patients,but CD 4+T / CD 8+T 1 in healthy controls. Significantly decreased serum level of IL-2 was found in SLE patients as compared to that in healthy controls( P 〈0. 01). Increased serum level of IL-6 was found in SLE patients as compared to that in healthy controls.( P 〈0. 05). Conclusions: The level of T cell subsets and Th1 / Th2 balance to be dominant in Th2 cell has important significance in the pathogenesis and disease activity of SLE.
出处 《内蒙古医科大学学报》 2015年第3期225-228,共4页 Journal of Inner Mongolia Medical University
基金 内蒙古自治区自然科学基金资助项目(200711020948)
关键词 红斑狼疮 系统性 白细胞介素-2 白细胞介素-6 lupus erythematosus systemic interleukin-2 interleukin-6
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参考文献15

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二级参考文献75

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