摘要
目的:观察SLE病人体内Th1/Th2及Tc1/Tc2比例失衡及其与疾病的关系。方法:SLE病人(活动期病人15例,稳定期病人20例)及正常人20例外周全血经PMA(20 ng/m l)和离子霉素(ionomyc in,1μmol/L)刺激4小时后,采用四色流式细胞术检测CD3+CD8-T细胞(主要为Th细胞)及CD3+CD8+T细胞(Tc细胞)胞浆内IFN-γ及IL-4的表达水平。血清抗dsDNA抗体检测采用ELISA法、血清免疫球蛋白和尿蛋白含量测定采用免疫速率散射比浊法。结果:SLE活动期病人Th细胞中IFN-γ的阳性百分率明显低于稳定期病人及正常人,Tc细胞中IFN-γ的阳性百分率明显低于正常人;IL-4的阳性百分率各组间无明显差异。稳定期病人仅Tc细胞IFN-γ的百分率明显低于正常人。血清抗dsDNA抗体(+)病人Th和Tc细胞IFN-γ的阳性百分率明显低于抗dsDNA(-)病人;免疫球蛋白(IgG,IgA,IgM中任何一种)异常增高的病人Th及Tc细胞中虽IL-4的阳性百分率无变化,但平均荧光强度明显高于免疫球蛋白水平正常的病人。Th及Tc细胞中IFN-γ和IL-4的表达水平与尿蛋白含量无明显关系。结论:SLE病人体内存在1型及2型细胞因子的异常表达,并与疾病活动程度存在一定的关系,值得进行深入研究。
Objective：To investigate the balance of Thl/Th2 and Tcl/Te2 in peripheral whole blood from SLE patients and find out the relationship between disease activity and the balance of type 1 and type 2 cytokines. Methods ：Peripheral blood celia from SLE patients（ active, n = 15, inactive, n = 20） were stimulated and normal subjects （n = 20） with phorbol myristate acetate and ionomycin（PMA/I） for 4 h, then IFN-＇y and IL-4 in CD3 ^＋ CD8 ^＋T cells（Th） and CD3 ^＋ CD8^＋T ceils （Tc） were detected by reeently developed intracytoplasmic cytokine-staining techniques, anti-dsDNA antibodies in serum was detected with enzyme linked immunosorbant assay（ELISA） and immunoglobulin in serum and protein in urine were analyzed with rate nephelometry. Results：In SLE patients with active disease ,the percent of iFN-γ-secreting Th ceils significantly increased compared with SLE patients with inactive disease and normal after in vitro PMA/I stimulation,the percent of IFN-γ-secreting Tc cells significantly increased compared with normal,but IL-4-seereting Th cells and Tc cells did not vary significantly. In SLE patients with inactive diseases, only the percent of IFN-γ-secreting Tc cells significantly increased compared with control. The percent of IFN-γ-secreting Th and Tc cells in patients with anti-dsDNA antibody（ ＋ ） in serum decreased significantly compared with patients with anti-dsDNA antibody（ - ）. Although the percent of IL-4-secreting Th and Te eelh in patients with aberrantly high level of immunoglobulin had no change, but the mean fluorescent intensify （MIF） of IL-4-secreting Th and Tc cells increased significantly compared with patients with normal level of immunoglobulin. There was no correlation between the percent of IFN-γ, or IL-4 in Th or Tc cells and the content of protein in patients＇urine. Conclusion ：The balance between type 1 and type 2 cytokine-secreting T cells is disrupted in SLE patients ,especially IFN-γ-secreting T cells, which is significantly in favour of Th2 cells. The level of IFN-γ or IL-4 in Th or Tc cells varied partly with disease activity, which deserved further study.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第2期168-171,179,共5页
Chinese Journal of Immunology
基金
国家"863"高技术研究发展计划基金资助项目(2002AA214091)
关键词
SLE
IFN-Γ
IL4
SLE
Intracytoplasmlc cytokines
IFN-γ
IL-4
