摘要
目的探讨替加环素对多重耐药鲍曼不动杆菌ade B基因表达水平的影响。方法收集2012年9月至2014年9月在海南省农垦三亚医院分离的57株多重耐药鲍曼不动杆菌,采用E-test（Epsilome test）法测定替加环素对多重耐药鲍曼不动杆菌最低抑菌浓度（minimal inhibitory concentration,MIC）,采用PCR扩增技术检测ade B、adeR、ade S外排泵基因分布情况,采用实时荧光RTPCR技术检测各基因相对表达水平。结果 57株多重耐药鲍曼不动杆菌MIC值为0.1~8μg/m L,计算MIC50=2μg/m L,MIC90=4μg/m L;其中敏感49株占86.0%,中介5株占8.8%,耐药3株5.2%。将57株细菌按照MIC的范围分为敏感性降低组38株和敏感组19株,敏感性降低组对替加环素敏感菌株30株占78.9%,敏感组对替加环素敏感菌株19株占100.0%,敏感组对替加环素敏感性显著高于敏感性降低组（P〈0.05）。所有菌株中,共有41株同时检测出ade B、adeR、ade S基因,占86.0%;敏感性降低组检测出ade B、adeR、ade S基因29株占70.7%,敏感组12株占63.2%,2组比较差异无统计学意义;荧光RT-PCR检测结果显示,敏感性降低组ade B基因相对表达量显著低于敏感组（P〈0.05）,两组adeR、ade S基因相对表达水平比较差异无统计学意义。结论 Ade B基因在降低多重耐药鲍曼不动杆菌对替加环素的敏感性方面发挥着重要的作用,但是可能还存在ade B以外基因参与调控对替加环素的耐药。
Objective To explore the adeB gene expression level impact of tigecycline for multidrug resistance Acinetobacter baumannii.Methods 57 strains multidrug resistance Acinetobacter baumannii were separated from September.2012 to September.2014.Epsilome test method was used to detect minimum inhibitory concentration of tigecycline against multi drug resistant Acinetobacter baumannii.PCR amplification detected adeB, adeR, adeS efflux pump gene distribution.Real-time fluorescent RT-PCR detection technology were used to detect relative expression level of each gene. Results 57 strains of multidrug-resistant Acinetobacter Bauman MIC value were 0.1-8μg/mL.The calculation of MIC50 =2μg/mL, MIC90 =4μg/mL. 49 strains sensitivity accounted for 86.0%, 5 intermediate strains accounted for 8.8%, 3 resistant strains accounted for 5.2%.57 strains of bacteria were divided into 38 strains lower sensitivity group and 19 strains sensitive group, with reduced susceptibility in lower sensitivity group 30 strains, accounting for 78.9%, sensitive group were all sensitive to tigecycline, accounting for 100%, for tigecycline susceptibility sensitive group was significantly higher than that of lower sensitivity group （ P 〈0.65 ） .41 strains were detected adeB, adeR, adeS gene in all strains, accounted for 86.0%, 29 strains were detected adeB, adeR, adeS genes in lower sensitivity group, accounted for 70.7%,12 strains were detected in sensitive group accounted for 63.2%, the difference between the two groups had no statistical significance.Fluorescent RT-PCR detection results showed that the relative expression of adeB gene with lower susceptibility group was significantly lower than that of sensitive group （P〈0.05）, relative expression levels of adeR and adeS gene in two groups had no significant difference.Conclusion AdeB gene plays an important role in tigecycline for multidrug resistance Acinetobacter baumannii, but may also exist outside of the adeB gene is involved in the regulation of resistance to tigecycline.
出处
《中国生化药物杂志》
CAS
2015年第5期47-49,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
替加环素
鲍曼不动杆菌
最低抑菌浓度
基因表达
tigecycline
Acinetobacter baumannii
minimal inhibitory concentration
gene expression
