摘要
目的探讨解毒祛瘀滋阴方联合糖皮质激素应用于系统性红斑狼疮小鼠的疗效和机制。方法 2012年4月—2014年5月选取60只MRL/lpr小鼠采用随机数字表法分为3组,模型组20只,西药组20只,联合组20只;同时选取20只健康昆明小鼠作为空白对照组。空白对照组和模型组给予0.9%氯化钠溶液灌胃;西药组给予0.5 g/ml泼尼松混悬液灌胃;联合组给予1.0 g/ml泼尼松混悬液联合解毒祛瘀滋阴汤剂。比较各组小鼠的死亡率、外周血淋巴细胞(PBLC)凋亡率、皮质醇水平和糖皮质激素受体(GR)mRNA表达水平。结果干预12周后,空白对照组、模型组、西药组、联合组小鼠分别死亡0只、11只(55%)、7只(35%)、3只(15%),差异有统计学意义(χ2=5.237,P<0.05)。培养24 h和48 h时,模型组和西药组PBLC凋亡率低于空白对照组(P<0.05);西药组和联合组高于模型组(P<0.05);联合组高于西药组(P<0.05)。各组小鼠皮质醇水平比较,差异有统计学意义(P<0.05);其中西药组和联合组高于空白对照组和模型组(P<0.05);联合组低于西药组(P<0.05)。各组小鼠GRαmRNA和GRβmRNA表达水平比较,差异有统计学意义(P<0.05);其中模型组、西药组和联合组低于空白对照组(P<0.05);西药组和联合组低于模型组(P<0.05);联合组高于西药组(P<0.05)。结论解毒祛瘀滋阴方联合糖皮质激素应用于系统性红斑狼疮小鼠可改善其死亡情况,疗效优于西药单独应用,机制可能与降低皮质醇水平、升高GR mRNA表达水平有关。
Objective To study the curative effect and mechanism of Jieduquyuziyinfang combined with glucocorticoid in treatment of mice with systemic lupus erythematosus. Methods 60 MRL/lpr mice were equally divided into 3 groups by random number table method： model group, western medicine group, and combined treatment group; 20 healthy Kunming mice were chosen as blank control group from April 2012 to May 2014. Mice in blank control group and model group received intragastric administration of 0. 9% sodium chloride solution; mice in western medicine group received intragastric administration of 0. 5 g/ml metacortandracin suspension; mice in combined treatment group received intragastric administration of 1.0 g/ml metacortandracin suspension and Jieduquyuziyin decoction. The rate of death, rate of PBLC apoptosis, levels of CORT and GR mRNA were compared among different groups. Results 12 weeks after treatment, 0 case in blank control group, 11 cases （55%） in model group; 7 cases （35%） in western medicine group and 3 cases （!5%） in combined treatment group mice died, there were significant differences in the rate of death among different groups （ X2 = 5. 237, P 〈 0. 05 ） . 24 h and 48 h after treatment, the rate of PBLC apoptosis in model group and western medicine group was significantly lower than that in blank control group, respectively; the rate of PBLC apoptosis in western medicine group and combined treatment group was significantly higher than that in model group （ P 〈 0. 05 ） ; the rate of PBLC apoptosis in combined treatment group was significantly higher than that in western medicine group （ P 〈 0.05 ） . There were significant differences in level of peripheral blood CORT among different groups of mice （P 〈 0.05）, level of peripheral blood CORT in western medicine group and combined treatment group was significantly higher than that in blank control group and model group, respectively （ P 〈 0.05 ） ; level of peripheral blood CORT in combined treatment group was significantly lower than that in western medicine group （ P 〈 0. 05 ） . There were significant differences in expression levels of GRct mRNA and GRI3 mRNA among different groups of mice （ P 〈 0. 05 ） ; expression levels of GRot mRNA and GRI3 mRNA in model group, western medicine group and combined treatment group were significantly lower than those in blank control group, respectively （ P 〈 0. 05 ） ; expression levels of GRa mRNA and GRβ mRNA in western medicine group and combined treatment group were significantly lower than those in model group, respectively （P 〈 0. 05） ; expression levels of GRa mRNA and GRI3 mRNA in combined treatment group were significantly higher than those in western medicine group （P 〈 0. 05 ） . Conclusion Jieduquyuziyinfang combined with glucocorticoid can reduce the rate of death among mice with systemic lupus erythematosus, the curative effect of which is better than that of western medicine, the mechanism may be related to the reduction effect of Jieduquyuziyinfang combined with glucocorticoid on CORT level and the enhancement effect of Jieduquyuziyinfang combined with glucocorticoid on GR mRNA expression level.
出处
《中国全科医学》
CAS
CSCD
北大核心
2015年第21期2589-2593,共5页
Chinese General Practice
基金
国家自然科学基金资助项目(81202673)
关键词
红斑狼疮
系统性
解毒剂(中药)
祛瘀
滋阴
方剂
糖皮质激素类
小鼠
Lupus erythematosus, systemic
Antidotic agents （ TCD ）
Removing blood stasis
Nourishing yin
Chinese medical formula
Glucocorticoids
Mice
