摘要
为挖掘重要的抗盐碱相关基因,进而阐述羊草(Leymus chinensis)耐盐碱胁迫的分子机制,采用荧光双向差异凝胶电泳(2D-DIGE)结合串联飞行时间质谱(MALDI TOF/TOF)方法,利用非盐碱条件下及Na_2CO_3处理下生长的羊草建立蛋白质表达谱,并进行差异蛋白质的分离鉴定。结果表明:2D-DIGE获得的74个差异蛋白点,其中蛋白质表达丰度上调的蛋白点44个,丰度下调点为30个,质谱鉴定显示其中33个蛋白质信息已知,参与了能量转换、代谢、光合作用、植物抗性和转录的过程。
To reveal the molecular mechanism of saline-alkaline tolerance from Leymus chinensis, the protein expression profile was constructed by 2D-DIGE and MALDI TOF/TOF. There were 74 protein spots presenting to express differentially after the total proteins were separated by 2D-DIGE. The 44 proteins of all presenting spots were up-regulated, and 30 proteins were down-regulated. Among the 74 protein spots, the annotations of the 33 differential expressed proteins were known. The expressed proteins were analyzed and compared by mass spectrum. Identified differential proteins took part in the energy exchange, metabolic processes of basal metabolism, photosynthesis, plant defense, and DNA synthesis, respectively. These works will be fundamental data to illuminate the molecular mechanism of plant defense to saline-al- kaline stress.
出处
《草地学报》
CAS
CSCD
北大核心
2015年第3期549-556,共8页
Acta Agrestia Sinica
基金
中央高校基本科研业务费专项资金项目(2572014CA21)
黑龙江省自然科学基金项目(C201343)
哈尔滨市科技创新人才研究专项资金项目(2013RFQXJ036)
"十二五"863计划现代农业技术领域项目(2011AA10020801)资助
