摘要
目的:探讨加减一阴煎对去势大鼠内皮功能紊乱的影响。方法:采用血管张力仪测定SD大鼠内皮依赖性舒张功能,扫描电镜观察胸主动脉内皮细胞形态,Western blotting技术检测内皮脂肪酶的表达,HE染色观察大鼠子宫内膜的形态。结果:加减一阴煎逆转了去势大鼠内皮依赖性舒张功能的降低;加减一阴煎减少了去势大鼠内皮细胞脂质的堆积,降低了去势大鼠内皮脂肪酶的表达水平;加减一阴煎对去势大鼠子宫湿重的影响不大。结论:加减一阴煎可改善去势大鼠血管内皮依赖性舒张功能,维持内皮细胞形态的完整。
AIM：To investigate the effect of Jia-jian-yi-yin decoction on endothelium-dysfunction in ovariecto-mized rats.METHODS：The ovariectomized rats were treated with Jia-jian-yi-yin decoction or turbid liquid of estradiol va-lerate for 8 weeks.The vascular ring tension was measured.Scanning electron microscopy and Western blotting were ap-plied to assess the function of endothelium-dependent dilation, aortic endothelial morphology and the expression of endothe-lial lipase.The pathologic sections were prepared to observe the effect of Jia-jian-yi-yin decoction on the uterus.RE-SULTS：In ovariectomized rats, the decrease in endothelium-dependent relaxation to acetylcholine （ ACh） was reversed to normal level, the endothelial morphology returned to normal without lipid accumulation and the endothelial lipase expression was decreased by Jia-jian-yi-yin decoction.Furthermore, no obvious change of the wet weight of uterine between the ovari-ectomized rats with or without Jia-jian-yi-yin decoction treatment was observed.CONCLUSION：Jia-jian-yi-yin decoction may have protective effects on endothelium-dependent vasodilation and aortic endothelial morphology in estrogen-deficient animals.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2015年第6期1008-1013,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81172525)
广东省科技厅项目(No.2012A032500002)
关键词
加减一阴煎
戊酸雌二醇
内皮依赖性舒张
内皮脂肪酶
Jia-jian-yi-yin decoction
Estradiol valerate
Endothelium-dependent dilation
Endothelial lipase
