摘要
目的观察白细胞介素1β和肿瘤坏死因子α对大鼠主动脉平滑肌细胞内皮脂肪酶表达的影响。方法采用贴块法原代培养大鼠主动脉平滑肌细胞,选用第3～5代细胞随机分组实验:对照组、不同浓度白细胞介素1β干预组和不同浓度肿瘤坏死因子α干预组。用酶联免疫吸附法、逆转录聚合酶链反应分别检测诱导后细胞内皮脂肪酶蛋白及mRNA表达水平。结果平滑肌细胞经不同浓度白细胞介素1β或肿瘤坏死因子α诱导24h后,内皮脂肪酶表达水平上升,与对照组比较差异有统计学意义(P<0.05),各浓度组间比较差异亦有统计学意义(P<0.05)。白细胞介素1β、肿瘤坏死因子α分别为30μg/L、50μg/L时内皮脂肪酶表达水平最高。结论白细胞介素1β、肿瘤坏死因子α均能诱导大鼠主动脉平滑肌细胞内皮脂肪酶表达。
Aim To investigate the effects of interleukin-1β（IL-1β） and tumor necrosis factor-α （TNF-α） on endothelial lipase （EL） in rat aortic smooth muscle cell （ASMC）. Methods The rat ASMC were obtained from thoracic aortas and cultured by tissue explant method. Passage 3 - 5 cells were used in experiment, and were randomly divided into 3 groups ： Group Ⅰ ： treated without intervention as the control ; Group Ⅱ ： the rat ASMC were treated in seromfree culture medium containing IL-1β （ 1 to 30 μg/L） for 24 hours ; Group Ⅲ ： TNF-α （ 5 to 50 μg/L） for 24 hours. The levels of EL were tested by the method of enzyme linked immunosorbent assay （ELISA） and reverse transcription polymerase chain reaction （RT-PCR）. Results Smooth muscle cells were treated with increasing concentrations of IL-1β, ranging from 1 μg/L to 30μg/L. The relative increases in EL protein and mRNA were greater at the highest concentrations （ 30μg/L） of IL-1β. There was a significant difference between the intervention group and the control group （ P 〈 0. 05 ）. There were significant differences among the intervention groups （ P 〈 0.05 ）. The relative increases in EL protein and mRNA were greater at the highest concentrations （50 μg/L） of TNF-α. There was a significant difference between the intervention group and the control group （ P 〈 0.05 ）. There were significant differences among the intervention groups （ P 〈 0.05 ）. Conclusion Smooth muscle cells were treated with increasing concentrations of IL-1β and TNF-α, then secreted protein and EL mRNA were noted.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2009年第3期181-184,共4页
Chinese Journal of Arteriosclerosis
基金
山西医科大学第二医院院内基金项目(20070102)
