摘要
目的：研究大豆异黄酮（soybean isoflavone, SI）对实验性糖尿病模型大鼠血脂调节和抗氧化能力的影响。方法将120只SD大鼠按随机数字表法分为正常对照组，模型组，大豆异黄酮40、80、160和320 mg/kg组，每组20只。除正常对照组外，其余各组大鼠腹腔注射60 mg/kg链脲佐菌素（STZ）制备大鼠糖尿病模型。大豆异黄酮40、80、160和320 mg/kg组腹腔注射相应浓度大豆异黄酮溶液，正常对照组和模型组腹腔注射等体积生理盐水，连续给药8周后取材检测。分别测定各组大鼠血糖浓度；检测血清中总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、天冬氨酸转氨酶（AST）、肌酸激酶（CK）、乳酸脱氢酶（LDH）水平及总抗氧化能力（T-AOC）；测定心肌组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性以及丙二醛（MDA）水平；采用苏木精-伊红（HE）染色观察心肌组织病理形态学改变。结果与模型组比较，大豆异黄酮80、160和320 mg/kg组大鼠血清中 AST[（104.25±24.92）U/L、（93.71±22.58）U/L、（88.26±23.80）U/L 比（127.65±38.17）U/L]、LDH[（954.7±153.5）U/L、（868.7±136.2）U/L、（834.1±146.3）U/L比（1097.6±184.2）U/L]、CK[（887.1±185.4）U/L、（831.9±182.8）U/L、（796.2±165.8）U/L比（973.6±211.4）U/L]活性降低（P＜0.05或P＜0.01），心肌组织中SOD [（9.96±2.05）U/mg、（10.47±2.32）U/mg、（11.06±2.29）U/mg比（8.72±1.70）U/mg]活性升高（P＜0.05或P＜0.01）、MDA[（5.98±1.35）nmol/mg、（5.47±1.42）nmol/mg、（5.16±1.53）nmol/mg 比（6.58±1.54）nmol/mg]水平降低（P＜0.05或P＜0.01）；大豆异黄酮160、320 mg/kg组大鼠血糖水平[（15.2±0.9）mmol/L、（14.8±0.8）mmol/L 比（18.6±1.2）mmol/L]降低（P＜0.05或 P＜0.01）；血清中 T-AOC[（10.15±2.76）U/L、（11.29±3.47）U/L比（7.95±2.26）U/L]、HDL-C水平[0.73±0.20）mmol/L、（0.78±0.22）mmol/L比（0.62±0.14）mmol/L]升高（P＜0.05），TC[（2.69±0.85）mmol/L、（2.43±0.76）mmol/L比（3.42±0.81）mmol/L]、TG[（1.36±0.40）mmol/L、（1.18±0.23）mmol/L比（1.70±0.53）mmol/L，]、LDL-C[（1.02±0.26）mmol/L、（0.95±0.28）mmol/L比（1.18±0.27）mmol/L]水平降低（P＜0.05或P＜0.01）；心肌组织中GSH-Px活性[（13.79±2.62）U/mg、（14.21±2.87）U/mg比（11.90±2.03）U/mg]升高（P＜0.05或P＜0.01）；大豆异黄酮160、320 mg/kg组心肌组织病理学改变明显改善。结论大豆异黄酮可有效降低糖尿病大鼠血糖水平，降低血脂，增强机体抗氧化酶系统活性、抑制氧化应激损伤，对糖尿病大鼠有保护作用。
Objective To investigate the effects of soybean isoflavone（SI） regulating plasma lipid and improving antioxidant ability in diabetic rats.Methods 120 SD rats were randomly devided into a normal group, a diabetes model group, a SI 40, 80, 160 and 320 mg/kg treated group, with 20 expermental animals in each group. Expect the normal group, the diabetic model was setup by intraperitoneal injecting STZ, and the drugs were given by intraperitoneal injection. At 0, 14, 28, 42, 56 days after experiment, the level of plasma glucose were determined. 8 weeks later, the content of TC, TG, LDL-C, HDL-C, the activity of AST, CK, LDHand the T-AOC in serum were determined; the activity of SOD, GSH-Px and the content of MDA in myocardial tissue were determined; and the histopathological changes of myocardial tissue were observed by HE staining. ResultsCompared with the diabetes model control group, the activity of AST in serum of SI 80, 160 and 320 mg/kg treated groups were significantly decreased（104.25 ± 24.92 U/L, 93.71 ± 22.58 U/L, 88.26 ± 23.80 U/Lvs. 127.65 ± 38.17 U/L;P〈0.05,P〈0.01）, the activity of CK were significantly decreased （887.1 ± 185.4 U/L, 831.9 ± 182.8 U/L, 796.2 ± 165.8 U/Lvs. 973.6 ± 211.4 U/L;P〈0.05,P〈0.01）, the activity of LDH were significantly decreased （954.7 ± 153.5 U/L, 868.7 ± 136.2 U/L, 834.1 ± 146.3 U/Lvs. 1 097.6 ± 184.2 U/L;P〈0.05,P〈0.01）; the activity of SOD in myocardial tissue were significantly increased（9.96 ± 2.05 U/mg, 10.47 ± 2.32 U/mg, 11.06 ± 2.29 U/mgvs. 8.72 ± 1.70 U/mg;P〈0.05,P〈0.01）, the content of MDA in myocardial tissue were significantly decreased（5.98 ± 1.35 nmol/mg, 5.47 ± 1.42 nmol/mg, 5.16 ± 1.53 nmol/mgvs. 6.58 ± 1.54 nmol/mg;P〈0.05, P〈0.01）. The plasma glucose level of SI （160 and 320 mg/kg） treated groups were significantly decreased（15.2 ± 0.9 mmol/L, 14.8 ± 0.8 mmol/Lvs. 18.6 ± 1.2 mmol/L;P〈0.05,P〈0.01）, the content of TC in serum were significantly decreased （2.69 ± 0.85 mmol/L, 2.43 ± 0.76 mmol/Lvs. 3.42 ± 0.81 mmol/L;P〈0.05,P〈0.01）, the content of TG were significantly decreased （1.36 ± 0.40 mmol/L, 1.18 ± 0.23 mmol/Lvs. 1.70 ± 0.53 mmol/L;P〈0.05,P〈0.01）, the content of LDL-C were significantly decreased （1.02 ± 0.26 mmol/L, 0.95 ± 0.28 mmol/Lvs. 1.18 ± 0.27 mmol/L;P〈0.05）, and the content of HDL-C were significantly increased （0.73 ± 0.20 mmol/L, 0.78 ± 0.22 mmol/Lvs. 0.62 ± 0.14 mmol/L;P〈0.05）, the T-AOC was significantly increased （10.15 ± 2.76 U/L, 11.29 ± 3.47 U/Lvs. 7.95 ± 2.26 U/L;P〈0.05）, and the activity of GSH-Px was significantly increased（13.79 ± 2.62 U/mg, 14.21 ± 2.87 U/mgvs. 11.90 ± 2.03 U/mg,P〈0.05,P〈0.01）. The histopathological changes of myocardial tissue in SI 160 and 320 mg/kg treated groups were significantly improved.Conclusions SI could effectively lower the plasma glucose level and the blood lipid, improve antioxidant ability, improve antioxidant ability, reduce the damage of free radical, and inhibit the histopathological changes, which suggesting that SI had protective effects on diabetic rats.
出处
《国际中医中药杂志》
2015年第7期626-631,共6页
International Journal of Traditional Chinese Medicine
关键词
大豆异黄酮
糖尿病
调节血脂
抗氧化
Soybean isoflavone
Diabetes
Regulating plasma lipid
Antioxidant
