摘要
为了快速大量得到鸡树条荚蒾苗木,尝试采用组培方法进行繁育,该试验以茎尖为外植体,研究了初代培养阶段不同培养基配方对其愈伤组织诱导以及不定芽诱导的影响。结果得出鸡树条荚蒾茎尖离体培养的培养方案为:选用带芽茎段经过消毒后,切割芽转入MS+KT0.6 mg/L愈伤组织诱导培养基上,产生愈伤组织后,将愈伤组织切块转入MS+KT0.5mg/L+IBA 0.2 mg/L或MS+KT1.0 mg/L+IBA0.2 mg/L的芽诱导培养基上,待产生大量不定芽时,以这些不定芽作为中间繁殖体,再进一步扩繁、生根,形成再生苗木。
In order to breed seedlings of Viburnum sargenti Koehne rapidly and largely, try to breed by using propagation methods in vitro. In the test, taking shoot-tips as explants, the effects of different media formulations on the callus induction and the bud induction were researched in the induction phase. The results got that the training program of shoot-tips culture of Viburnum sargenti in vitro was that： the stems with buds after disinfection, the buds were cut and seeded into the callus induction medium of MS＋KT 0.6 mg/L, when produced callus, the callus were cut pieces and seeded into the bud induction medium of MS＋KT 0.5 mg/L＋IBA 0.2 mg/L or MS＋KT 1.0 mg/L＋IBA 0.2 mg/L, when produced a large number of adventitious buds, took these buds as middle propagates for further propagation and rooting, and become full seedlings.
出处
《吉林农业科技学院学报》
2015年第2期1-3,共3页
Journal of Jilin Agricultural Science and Technology University
关键词
鸡树条荚蒾
培养基配方
愈伤组织诱导
芽诱导
Viburnum sargenti Koehne
media formulations
callus induction
bud induction
