摘要
目的研究沙格雷酯对大鼠颈动脉球囊损伤后新生内膜的抑制作用,并通过监测增殖细胞核抗原(PCNA)的表达间接比较血管平滑肌细胞(VSMCs)的增殖状态。方法采用前瞻性随机对照研究设计,将24只8周龄SPF级健康雄性SD大鼠按随机数字表法分为空白对照组、沙格雷酯组和氯吡格雷组,每组8只。所有大鼠从术前第7 d开始持续饲喂高脂饮食,空白对照组不饲喂药物,另外2组每天分别饲喂沙格雷酯(100 mg/kg)和氯吡格雷(20 mg/kg)。通过Forgarty导管球囊损伤大鼠颈总动脉。术后第14 d时处死大鼠,取材球囊损伤的颈动脉标本进行组织形态学分析和PCNA免疫组织化学分析,计算内膜/中膜厚度比和面积比,计算PCNA阳性细胞率和吸光度。结果沙格雷酯组和氯吡格雷组分别与空白对照组比较,平均内膜厚度、平均内膜面积、内膜/中膜厚度比、内膜/中膜面积比、PCNA吸光度和阳性细胞率均显著降低(P<0.001);沙格雷酯组与氯吡格雷组比较,以上指标比较差异均无统计学意义(P>0.05)。3组的平均中膜厚度和面积比较差异均无统计学意义(P>0.05)。结论沙格雷酯和氯吡格雷均能显著降低大鼠颈动脉球囊损伤后内膜的厚度和面积以及PCNA的吸光度和阳性细胞率。
Objective To assess the inhibitory ability of sarpogrelate on neointimal hyperplasia of carotid artery in rat balloon-injuried model, and to compare the proliferation of vascular smooth muscle cell （VSMC） by monitoring the expression of proliferative cell nuclear antigen （PCNA）. Methods Twenty-four male SD rats （SPF, 8 weeks） were allocated prospectively and randomly into 3 groups： blank group, sarpogrelate group, and clopidogrel group. Each group included 8 rats. All the rats were fed high-fat diet for I week before the operation. No drug was fed in the blank group, and sarpo- grelate 100 mg/（kg· d） or dopidogrel 20 mg/（kg ~ d） was fed in the sarpogrelate group or clopidogrel group respectively. The carotid artery of rat was dilated by the Forgarty balloon catheter. The rats were killed 2 weeks later and the samples were got in the balloon-injuried carotid arteries. Histomorphological analysis and immunohistochemical analysis were pro- ceeded. The thickness ratio and area ratio of intima and media, the ratio of PCNA positive cells and PCNA absorbance were calculated among the three groups. Results Compared with the blank group, the average intimal thickness, average intimal area, thickness ratio of intima and media, area ratio of intima and media, PCNA absorbance, and ratio of PCNA positive cells were significant decreased in the sarpogrelate group （P〈0.001） and the clopidogrel group （P〈0.001）, but which had no significant differences between the sarpogrelate group and the clopidogrel group （P〉0.05）. There was no significant difference in the average media thickness or area among the 3 groups （P〉0.05）. Conclusion Sarpogrelate and clopidogrel could significantly reduce the thickness or area ofintima, the absorbance of PCNA and the ratio of PCNA positive cells.
出处
《中国普外基础与临床杂志》
CAS
2015年第5期571-575,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
沙格雷酯
氯吡格雷
内膜增生
颈动脉
增殖细胞核抗原
Sarpogrelate
Clopidogrel
Intimal hyperplasia
Carotid artery
Proliferative cell nuclear antigen
