摘要
从茶树内生真菌筛选产漆酶的菌株,分析不同营养因素和培养条件对菌株漆酶酶活力的影响。采用6种显色底物的平板初筛和酶活测定的复筛方法,从15株茶树内生真菌菌株中筛选获得1株产漆酶酶活较高的菌株CSN-4。单因素分析结果显示,液态发酵条件下菌株CSN-4适宜的主要培养基成分是麸皮和蛋白胨;菌株CSN-4分别在麸皮30 g/L、蛋白胨2.5 g/L、CuSO4·5H2O 0.015 g/L和茶水6 g/L时发酵产漆酶酶活最高。发酵条件试验结果表明,菌株CSN-4分别在接种量为6个菌饼(直径6 mm)、装液量60mL/250mL、pH 4.8、摇床转速120 r/min,培养温度为28℃时产漆酶酶活较高。在培养基中添加麸皮和茶水对菌株CSN-4产漆酶有明显的促进作用。经过培养基成分及培养条件优化后,菌株CSN-4产漆酶酶活显著升高,达到2 417 U/L。
A laccase-producing fungal strain CSN-4 was screened from 15 endophytic fungi isolated from healthy tea （ Camellia sinensis） leaves with PDA medium plate amended with six coloration substrates for initial screening and re- screening by checking their laccase activity, and the effects of different nutritional factors and cultural condition on lacease activity of the strain were analyzed. The results of single factor analysis showed that the main medium compo- nents under liquid fermentation suitable for strain were wheat bran and peptone; laccase activity of strain CSN-4 reached the peak when fermented with 30 g/L of wheat bran, 2.5 g/L of peptone, 0. 015 g/L of CuSO4 · 5H20 and 6 g/L of tea infusion, respectively. The results of the experiments on fermentation conditions showed that when the fermentation was inoculated with six strain agar plugs （6 mm in diameter）, liquid filling amount at 60 mL/250 mL, pH 4. 8, shaking at 120 r/min, and culture temperature at 28 ~C, the laecase activity produced by the strain reached a fairly high. The addition of wheat bran and tea infusion had a significant improvement on laccase production by CSN- 4. The laccase activity of CSN-4 significantly ascended and reached as high as 2 417 U/L after the optimization of cul- ture medium compositions and culture conditions.
出处
《微生物学杂志》
CAS
CSCD
2015年第2期78-84,共7页
Journal of Microbiology
基金
福建省科技厅重点项目(2012N0013)
福建省自然科学基金项目(2012J01122)
关键词
茶树
内生真菌
漆酶
培养基成分
发酵条件
Camellia sinensis
endophytic fungi
laccase
medium components
fermentation condition
