摘要
选用目前主要推广的甘薯品种‘烟薯25’、‘商薯19’和‘徐薯22’为材料,研究了甘薯脱毒快速繁殖及驯化移栽。结果表明,秋天刚收获的薯块在36℃条件下催芽,10~15 d后芽苗可长到10 cm以上；剥取带有1~2个叶原基的茎尖进行培养,3个供试品种植株再生率均达到90%以上；脱毒试管苗炼苗2~3 d后直接栽植于塑料大棚,移栽成活率达95%以上；移栽5~6周后茎蔓可长到30~40 cm以上,即可剪切茎蔓扦插进行再繁殖。
The method on production, acclimatization and transplantation of virus-free plantlets was studied by using cultivars‘Yanshu 25’,‘Shangshu 19’ and‘Xushu 22’ of sweet potato （Ipomoea batatas）. The results in-dicated that the seedlings could grow more than 10 cm after a germination acceleration at 36℃for 10–15 d. Then, stem tips with 1–2 leaf primordia were excised and cultured on the induction medium, the plantlet regen-eration rates were above 90%in the three cultivars. The virus-free plantlets in tubes were acclimated for 2–3 d and subsequently transferred to soil in plastic greenhouse, and the transplanting survival rates of the three culti-vars were over 95%. After having been transplanted for 5–6 weeks, the lateral branches reached 30–40 cm in length, and they can be used for propagation in plastic greenhouse.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第4期455-458,共4页
Plant Physiology Journal
基金
山东省薯类产业创新团队遗传育种岗位(SDAIT-10-011-03)
关键词
甘薯
热处理
茎尖培养
脱毒苗
驯化移栽
sweet potato
heat treatment
stem tip culture
virus-free plantlet
acclimatization and transplantation
