摘要
选取黄河流域棉区的 72个抗病品种和长江流域棉区的 2 9个抗病品种 ,利用AFLP技术对其进行了遗传多样性分析。结果 2 0对具有多态性的AFLP引物组合在黄河流域棉区的 72个品种和长江流域棉区的 2 9个品种上分别扩增出 2 0 0条和 12 7条多态性带 ,利用SPSS(11 0 )软件计算得到品种之间的平均欧氏距离为 4 .35 6 (黄河流域棉区 )和 4 391(长江流域棉区 )。在阈值取 15 2时 ,可以将黄河流域棉区的 72个品种划分为 4个类群 (theHuanghevalleygroups ,abbreviateHVGs) ,即HVG1、HVG2、HVG3和HVG4 ,分别包括 2 7、19、10和 16个品种 ;长江流域棉区的 2 9个品种被划分为 4个类群 (theChangjiangvalleygroups ,CVGs) ,即CVG1、CVG2、CVG3和CVG4 ,分别包括 14、4、5、和 6个品种。通过比较两棉区品种成对欧式距离的最大值、最小值、平均值及成对欧氏距离的区间分布和累积百分率 ,表明来自黄河。
Genetic diversity among 101 cottons varieties,including 72 cultivars from the Huanghe valley and 29 cultivars from the Changjiang valley,was investigated using AFLP markers.In total,20 primer combinations revealed 200 polymorphic bands among the Huanghe valley cottons and 127 polymorphic bands among the Changjiang valley,respectively.Euclidean distance values were calculated using SPSS(11.0) software.Average Euclidean distance value was 4.356(the Huanghe valley) and 4.391(the Changjiang valley),respectively.When the value was 15.2,72 varieties from the Huanghe valley were divided into four groups(the Huanghe valley groups,abbrevate HVGs),including HVG1(27),HVG2(19),HVG3(10) and HVG4(16).29 varieties from the Changjiang valley were classified into four groups( the Changjiang valley groups,abbreviate CVGs),including CVG1(14),CVG2(4),CVG3(5) and CVG4(6).Compared with Euclidean distance matrices and frequency distribution of pairwise Euclidean distances of cottons from the Huanghe and Changjiang valleys,it was showed that there was similar genetic diversity between the cotton cultivars from Huanghe valley and those from Changjiang valley.
基金
教育部重点科技项目 (编号 :0 10 15 )
国家留学归国人员基金
河北省自然科学基金重点项目资助 (编号 :3 0 1167)~~
关键词
棉花
抗病性
遗传多样性
AFLP
cotton
disease resistance
genetic diversity
AFLP
