摘要
比较研究休哈塔假丝酵母单菌发酵、休哈塔假丝酵母和酿酒酵母两步发酵、休哈塔假丝酵母和酿酒酵母同步发酵3种发酵模式下对混合糖(葡萄糖和木糖)的利用情况,结果显示2种酵母同步发酵能够有效解除葡萄糖的抑制,加快木糖利用速率。采用Plackett-Burman实验、最陡爬坡实验和中心组合设计,对2种酵母混菌利用戊糖己糖发酵生产乙醇的培养基进行优化,得到最优培养基条件:硫酸铵9.09 g/L、磷酸二氢钾8.96 g/L、氯化钙0.34 g/L,在此条件下发酵得到乙醇产量为21.71 g/L,较优化前(19.85 g/L)提高了9.4%;混菌在优化后的培养基下进行发酵,酵母浊度OD由12.12增长到了21.87,木糖利用率由优化前的82%增长到优化后的93%。
Mixed sugars （glucose and xylose）consumption patterns by the following three fermentation methods, i. e. , single bacterium fermentation by Candida shehatae, successive fermentation by C. shehatae and Saccharomyces cerevisiae, as well as synchronization （fermentation by these two）, were investigated. The results showed that the fermentation by synchronization pattern can effectively terminate the inhibition of glucose, and accelerate the xylose utilization at the same time. The fermentation medium of mixed yeast was optimized by Plackett-Burman design, steepest climbing experimental design, and central composite design. The optimal culture conditions were （NH4）2SO4 9.09 g/L, KH2PO4 8.96 g/L and CaC12 0.34 g/L. Under the above conditions of fermentation, the ethanol yield was 21.71 g/L, which is 9.4 % higher than before. Under the optimized medium, the yeast turbidity （OD） increased from 12.12 to 21.87, and the conversion of xylose also increased from 82 % to 93 %.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2015年第2期111-116,共6页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(30871992)
江苏省科技支撑计划项目(BE2010732)
江苏高校优势学科建设工程资助项目(无编号)
关键词
混合糖
混菌发酵
乙醇
培养基
优化
mixed sugar
fermentation of mixed yeasts
ethanol
medium
optimization
