摘要
目的探讨线性探针(MTBDR plus)技术和传统基因检测技术在耐药结核病诊断中的可靠性和及时性。方法同时采用MTBDR plus技术、核酸扩增检测技术和传统药敏实验方法对2014年1月至10月期间我院收治的246例涂阳肺结核患者的痰标本进行利福平和异烟肼耐药性的检测。以传统药敏实验检测结果为金标准,分析MTBDR plus技术和核酸扩增检测技术诊断耐药结核病的准确性,并比较MTBDR plus技术和核酸扩增出报告时间。结果 MTBDR plus技术检测所得利福平耐药结核病发生率、异烟肼耐药结核病发生率和耐多药结核病发生率分别为5.28%、7.32%和3.66%,和传统药敏实验检测所得的5.69%、6.91%和3.66%比较差异均无统计学意义(P>0.05)。核酸扩增检测技术检测所得利福平耐药结核病发生率、异烟肼耐药结核病发生率和耐多药结核病发生率则均低于传统药敏实验检测所得结果,差异有统计学意义(P<0.05)。MTBDR plus技术检测利福平耐药、异烟肼耐药和耐多药的敏感度、Kappa值均高于核酸扩增检测技术,差异有统计学意义(P<0.05)。MTBDR plus技术平均出报告时间为(1.26±0.58)d,显著低于核酸扩增的时间(8.89±1.08)d,差异有统计学意义(P<0.05)。结论线性探针技术较传统基因检测技术可更及时、准确地诊断耐药结核病。
Objective To study the reliability and timeliness of linear probe technique （MTBDR plus） and traditional genetic testing technique （TGTT） in diagnosing drug-resistant tuberculosis （TB）. Methods MTBDR plus, nucleic acid amplification testing technique and traditional drug susceptibility experiment （TDSE） method were used at the same time in the rifampicin and isoniazid resistance testing of sputum specimens of 246 smear-positive pulmonary tuberculosis patients in our hospital from January, 2014 to October, 2014. Taken the TDSE test results as the gold standard to analyze the accuracy of MTBDR plus and TGTT diagnosing drug-resistant TB and compare their report time. Results The incidence of Rifampin resistant TB, isoniazid resistant TB and mul- tidrug resistant TB tested by MTBDR plus technique were 5.28%, 7.32% and 3.66%, respectively, which had no statistical significance compared with the incidence tested by TDSE that were 5.69%, 6.91% and 3.66%, respectively （P〉0.05）. The incidence of Rifampin resistant TB, isoniazid resistant TB and multidrug resistant TB tested by TGTT lower than by TDSE, and the difference had statistically significant （P〈0.05）. The accuracy and Kappa value of diagnosing Rifampin resistance TB, isoniazid resistance TB and muhi-drug resistance TB by MTBDR plus was higher than TGTT, and the difference had statistically significant （P〈0.05）. The average report time of MTBDR plus was （1.26±0.58） d, which was significantly less than （8.89±1.08） d of TGTT, and the difference had statistically significant （P〈0.05）. Conclusions Compared with the TGTT, MTBDR plus technique can more timely and accurately diagnose drug-resistant TB.
出处
《实验与检验医学》
CAS
2015年第2期150-153,共4页
Experimental and Laboratory Medicine
关键词
线性探针技术
传统基因检测技术
耐药结核病
诊断
可靠性
及时性
Linear probe technique
Traditional genetictesting technology
Drug-resistant TB
Diagnosis
Reliability
Timeliness
