摘要
目的检测临床非小细胞肺癌(Non-small cell lung cancer,NSCLC)病人组织中TRIM25、PKM2蛋白的表达情况,探讨它们的关联性及意义。方法应用组织免疫荧光法,检测TRIM25、PKM2蛋白在60例NSCLC组织和20例癌旁边正常肺组织(>5 cm)表达情况,同时运用Western blotting方法检测TRIM25、PKM2在10例新鲜NSCLC组织中表达情况,并结合标本的临床病理特征进行分析。结果 TRIM25在60例非小细胞肺癌组织中的阳性表达率45%,而20例癌旁正常肺组织中仅为10%(P=0.005)。TRIM25在腺癌中阳性表达率为28.6%,而在鳞癌中为59.4%(P=0.017)。TRIM25被发现与TNM分期、淋巴结转移之间呈正相关性(P<0.05)。PKM2在60例非小细胞肺癌组织中表达率为73.3%,而在20例癌旁正常肺组织中为30%(P=0.001)。PKM2在腺癌的表达为57.1%,而在鳞癌中为87.5%(P=0.008)。通过关联性分析TRIM25和PKM2存在相关性(P=0.026)。Western blot结果表明当TRIM25表达升高时PKM2表达增长放缓。结论 TRIM25、PKM2蛋白可能同时参与到NSCLC发生发展过程中,存在负相关性。
Objective To investigate the expression of tripartite- motif protein 25（TRIM25） and pyruvate kinase M2（PKM2）protein in non- small cell lung cancer（NSCLC） and explore their role in the occurrence and progression of NSCLC. Methods The expressions of TRIM25 and PKM2 protein were detected in 60 NSCLC specimens and 20 adjacent normal lung tissue（5cm from the lesions） with immunofluorescence histochemical method and in 10 fresh specimens of NSCLC with Western blotting. The results were analyzed in relation with the clinicopathological features of the patients. Results The positivity rates of TRIM25 expression was 45% in the 60 lung carcinoma specimens, significantly higher than that in the 20 normal lung tissues（10%, Ρ=0.005）. TRIM25 protein was expressed in 28.6% of lung adenocarcinoma tissues and in 59.4% of squamous carcinoma tissues（Ρ =0.017）. TRIM25 protein expression was positively correlated with the TNM stages and lymph node metastasis of NSCLC（Ρ0.05）. The expressions of PKM2 protein in 60 cases of lung carcinoma was 73.3%,while in 20 cases of normal lung tissues the expressions was 30%（Ρ =0.001）. The positivity rates of PKM2 expression differed significantly between lung adenocarcinoma and squamous carcinoma（57.1% vs 87.5%, Ρ=0.008）. An inverse correlation was noted between TRIM25 and PKM2 expressions（Ρ =0.026）. Conclusion TRIM25 and PKM2 protein may participate in the occurrence and progression of NSCLC, and their expressions are inversely correlated.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2015年第3期437-441,共5页
Journal of Southern Medical University
基金
重庆市卫生局医学科研重点项目
