摘要
为了防治石河子一串红花叶病,采用提取dsRNA/RNA、RT-PCR、克隆和序列分析对病原进行了检测鉴定。结果表明:从表现花叶的S4分离物中提取dsRNA,得到大小约4700和6300 bp的片段,以此dsRNA为模板,用蚕豆病毒属(Fabavirus)的兼并引物进行RT-PCR,扩增得到1条约390 bp片段特异性条带,序列测定该片段大小为391nt,blast比对分析与蚕豆萎蔫病毒2号(BBWV2)的同源性最高,表明S4分离物为BBWV2。RNA1基因组全序列测定及分析结果表明,基因组大小为5957nt(不含poly A),仅编码1个开放阅读框(ORF),与已报道BBWV2的RNA1序列同源性为78.4%-100.0%。
In order to control the virus diseases of Salvia splendens,.the virus species associated with mosaic symptom on it were identified by extraction of dsRNA,RT-PCR and sequence analysis.The results showed that ds RNA fragments of 4700 bp and6300 bp were extracted from S4 isolate.A 390 bp fragment was amplified from the dsRNA by polymerase chain reaction（PCR）using degenerate primers of Fabavirus.The RT-PCR products were cloned and the positive clone was subsequently sequenced.The segment size is 391 nt.Blast analysis revealed that it has the highest similarity with Bean Broad Wilt Virus 2（BBWV2）,showing that S4 isolate was BBWV 2.The sequencing of RNA1 genome in S4 isolate showed that it is 5957 nt containing an open reading frame（ORF）,with 78.4%-100% similarity with the RNA1 of BBWV2.
出处
《石河子大学学报（自然科学版）》
CAS
2015年第1期67-71,共5页
Journal of Shihezi University(Natural Science)
