摘要
本文旨在优化金银花多糖的复合酶法提取工艺,并评价金银花多糖的抗氧化活性。以不同复合酶(纤维素酶、果胶酶、木瓜蛋白酶)配比、液料比、pH、酶解温度、酶解时间为试验因素,以金银花多糖得率为考察指标,正交试验筛选复合酶法提取的最佳工艺条件;采用自由基清除能力体系评价金银花多糖的抗氧化活性。结果表明,复合酶最佳配比为纤维素酶2.0%,果胶酶2.0%,木瓜蛋白酶0.5%;复合酶酶解金银花提取多糖的工艺条件为液料比25:1(m L/g)、pH为4.5、酶解温度50℃、酶解时间60 min,在此条件下金银花多糖得率为12.36%;金银花多糖具有较强的抗氧化活性,对DPPH和O2-·自由基的半数抑制浓度分别为0.811 mg/m L、1.363 mg/m L,但与维生素C比较,抗氧化活性较弱。金银花多糖提取工艺方便可行,得率较高,提取到的多糖具有较强的抗氧化活性。
In order to optimize the compound enzymatic extraction technology of polysaccharide from Lonicera japonica and and investigate its antioxidant activity. The experiments used polysaccharides extraction rate as response value, and used enzymatic hydrolysi （cellulose, pectinase, papain）, the ratio of water to material, pH, extraction temperature and extraction time as experimental factors to optimize the polysaccharide extraction conditions from Lonicera japonica. Antioxidant activity of polysaccharides was measured by DPPH and O2-· free radical elimination method. The best ratio of compound enzyme was 2.0% cellulase, 2.0% pectinase, 0.5% papain. The optimum conditions were as follows, the ratio of water to feedstock was 25 mL/g, pH4.5, extraction temperature was 50℃, extraction time was 60 min. Under the optimal conditions, the experimental extraction rate was 12.36%. IC50 of DPPH and O2-· were 0.811 mg/mL and 1.363 mg/mL, respectively. Antioxidant activity of sample polysaccharides was weaker than those of vitamin C. The optimum compound enzymatic extraction technology of the polysaccharides from Lonicera japonica was convenient and feasible, the polysaccharides extraction rate was higher than other extraction methods, and the extracted polysaccharides had good antioxidant activity.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2014年第5期646-650,共5页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
金银花
多糖
复合酶
酶法提取
抗氧化活性
Lonicera japonica
polysaccharide
compound enzymes
enzymatic extraction
antioxidant activities
